Recombinant Human Wnt-4 Protein

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Wnt-4 Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The typical ED50 for this effect is 20-100 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Wnt-4 protein
Ser23-Arg351
Accession #
N-terminal Sequence
Ser23
Structure / Form
Monomer
Protein/Peptide Type
Recombinant Proteins
Gene
WNT4
Purity
>60%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
39.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
42-80 kDa, reducing conditions
Publications
Read Publications using
6076-WN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Purity
>60%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-4 Protein

  • protein Wnt-4
  • SERKAL
  • wingless-type MMTV integration site family, member 4
  • Wnt4
  • Wnt-4

Background

Wnt-4 is a 38-42 kDa member of the Wnt family of secreted glycoproteins, which act as short-range signaling molecules via Frizzled receptors and a cascade of intracellular signals in vertebrate embryogenesis (1-2). Human Wnt-4 is synthesized as a 351 amino acid (aa) precursor with a 22 aa signal sequence and a 329 aa mature chain. The mature chain contains two potential sites for N-linked glycosylation. Relative to other members of the Wnt family, Wnt-4 contains 83 conserved aa, including 21 cysteines (1). Mature human Wnt-4 shares 99%, 98% and 99% aa sequence identity with mature mouse, rat and canine Wnt-4, respectively. Wnt-4 has been shown to play a critical role in the development of the reproductive system and in the formation of the kidneys, adrenals, pituitary gland, and mammary tissues (3-6). In the development of the reproductive system, Wnt-4 expression is down‑regulated in the developing gonad after E11.5, although it persists in the developing ovary (2, 6). Targeted deletion of Wnt-4 results in masculinization of XX mice, with rudimentary development of the masculine internal (Wolffian) ducts and degeneration of the female (Mullerian) reproductive tract (2, 6). In addition to its involvement in urogenital development, Wnt-4 is also expressed in the perichondrium of the long bones (7), and promotes osteoblast differentiation (8). Wnt-4 may also be associated with abnormal proliferation in human breast tissue (9). In humans, mutations in Wnt-4 are the cause of SERKAL syndrome, a syndrome consisting of female to male sex reversal, renal, adrenal, and lung dysgenesis, and developmental defects (3), and Rokitansky-Kuster-Hauser syndrome, which is characterized by utero-vaginal atresia in otherwise phenotypically normal females with normal 46, XX karyotype (10).

  1. Gavin, B.J. et al. (1990) Genes Dev. 4:2319.
  2. Jordan, B.K. et al. (2001) Am. J. Hum. Genet. 68:1102.
  3. Mandel, H. et al. (2008) Am. J. Hum. Genet. 82:39.
  4. Bernard, P. and V.R. Harley (2007) Int. J. Biochem. Cell Biol. 39:31.
  5. Kuulasmaa, T. et al. (2008) Horm. Metab. Res. 40:668.
  6. Vainio, S. et al. (1999) Nature 397:405.
  7. Gao, X. (2004) Genes Dev. 18:2404.
  8. Chang, J. et al. (2007) J. Biol. Chem. 282:30938.
  9. Huguet, E.L. et al. (1994) Cancer Res. 54:2615.
  10. Philibert, P. et al. (2008) J. Clin. Endocrinol. Metab. 93:895.

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Publications for Wnt-4 (6076-WN)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(6)
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Filter By Species
Human
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using 6076-WN Applications Species
N Yucer, M Holzapfel, T Jenkins Vo, L Lenaeus, L Ornelas, A Laury, D Sareen, R Barrett, BY Karlan, CN Svendsen Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium Sci Rep, 2017;7(1):10741. 2017 [PMID: 28878359] (Bioassay, Human) Bioassay Human
Identification of proliferative and mature ?-cells in the islets of Langerhans Nature, 2016;535(7612):430-4. 2016 [PMID: 27398620] (Bioassay, Human) Bioassay Human
Kilander M, Dahlstrom J, Schulte G Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal, 2014;26(9):1943-9. 2014 [PMID: 24873871] (Bioassay, Human) Bioassay Human
Garcia-Castro B, Alvarez-Zavala M, Riveros-Magana A, Ortiz-Lazareno P, Ratkovich-Gonzalez S, Hernandez-Flores G, Bravo-Cuellar A, Jave-Suarez L, Aguilar-Lemarroy A Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines. BMC Cancer, 2013;13(0):557. 2013 [PMID: 24274766] (Bioassay, Human) Bioassay Human
Thrasivoulou C, Millar M, Ahmed A Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways. J Biol Chem, 2013;288(50):35651-9. 2013 [PMID: 24158438] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol WNT4
Uniprot