Recombinant Human UCH-L5/UCH37 Protein, CF

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Summary
Product Discontinued
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    • Catalog Number
      E-327
    • Availability
      Product Discontinued

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Recombinant Human UCH-L5/UCH37 Protein, CF Summary

Details of Functionality

Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human UCH-L5 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)

Source
E. coli-derived human UCH-L5/UCH37 protein
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UCHL5
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
E-327 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 1 mM TCEP, 10% (v/v) Glycerol,
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UCH-L5/UCH37 Protein, CF

  • CGI-70
  • EC 3.4.19.12
  • INO80 complex subunit R
  • INO80R
  • ubiquitin carboxyl-terminal esterase L5
  • ubiquitin carboxyl-terminal hydrolase isozyme L5
  • ubiquitin carboxyl-terminal hydrolase L5
  • Ubiquitin C-terminal hydrolase UCH37
  • Ubiquitin thioesterase L5
  • UCH37
  • UCH37CGI-70
  • UCHL5
  • UCH-L5

Background

Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases (known as the peptidase C12 family) that catalyze the hydrolysis of amides, esters and thioesters from the c-terminal end of Ubiquitin. Human UCH-L5 (also known as UCH37) is 329 amino acids long with a predicted weight of 38 kDa, and shares 97% sequence identity with its mouse ortholog. UCH-L5 is found associated with the 19S regulatory complex of the 26S proteasome, where it functions to cleave K48-linked poly-Ubiquitin chains from Ubiquitinated substrates. UCH-L5 is also found as a component of the INO80 complex, where its activity is regulated by a mechanism involving ADRM1.

  1. Burgie S.E., et al. (2012) Proteins 80: 649
  2. Yao T., et al. (2006) Nat. Cell Biol. 8: 994
  3. Yao T., et al. (2008) Mol. Cell 31: 909

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E-327
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Publications for UCH-L5/UCH37 (E-327)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioassay
(5)
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Human
(5)
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Bioinformatics

Gene Symbol UCHL5
Uniprot