Recombinant Human Ubiquitin Agarose Protein, CF Summary
Details of Functionality
Ubiquitin Agarose is ideal for the enrichment of known Ubiquitin-interacting proteins as well as the discovery of novel Ubiquitin-interacting proteins. We recommend equilibrating the resin by washing with 5-10 mL of your desired aqueous buffer.
12 months from date of receipt, 2 to 8 °C as supplied.
3 months, 2 to 8 °C under sterile conditions after opening.
Supplied as a solution in 20% Ethanol.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human Ubiquitin Agarose Protein, CF
UBB ubiquitin B
Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).
Ubiquitin is covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for affinity binding of Ubiquitin-activating (E1) enzyme, Ubiquitin-conjugating (E2) enzymes, Ubiquitin ligases (E3s), Ubiquitin C-terminal hydrolases (UCHs), and other proteins/enzymes that have an affinity for Ubiquitin.
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