Recombinant Human Ubiquilin 1 Tandem UBA (TUBE2) Agarose, CF Summary
Details of Functionality
Ubiquilin-1 Tandem UBA (TUBE2) agarose is ideal for the enrichment of known TUBE2-interacting proteins as well as the discovery of novel TUBE2-interacting proteins. We recommend equilibrating the resin by washing with 5-10 mL of your desired aqueous buffer.
3 months from date of receipt, 2 to 8 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
Supplied as a solution in Ethanol and water.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human Ubiquilin 1 Tandem UBA (TUBE2) Agarose, CF
Protein linking IAP with cytoskeleton 1
Ubiquilin 1 is a ubiquitously expressed member of the UBL-UBA family of Ubiquitin-binding proteins that is 589 amino acids (aa) in length and has a predicted molecular weight of 62.5 kDa (1). Human Ubiquilin 1 shares 88% aa sequence identity with the mouse and rat orthologs. It contains an N-terminal Ubiquitin-like (UBL) domain and a C-terminal Ubiquitin-associated (UBA) domain (2). The UBA domain is able to interact with mono- and poly-Ubiquitin chains, with a similar affinity for Lys48- and Lys63-linked chains (3,4). In contrast, the UBL domain interacts with Ubiquitin-interacting motif (UIM)-containing proteins, such as S5a/Angiocidin, a 19S Proteasome component (5). It is thought that Ubiquilin 1 may act as a molecular shuttle, bringing poly-ubiquitinated proteins to the 26S Proteasome by simultaneously binding poly-Ubiquitin via its UBA domain and the 19S Proteasome via its UBL domain (5). Ubiquilin 1 also binds two endoplasmic reticulum (ER)-localized proteins, Herp and Erasin, and may function in the ER-associated degradation pathway (6,7). It has been reported to co-precipitate with LC3/MAP1LC3A/Apg8p3, an autophagosomal marker, suggesting that it may play a role in autophagy (8,9). Single nucleotide polymorphisms in the UBQLN1 gene have been linked to Alzheimer's disease (AD) (10). Furthermore, Ubiquilin 1 may regulate the processing of APP, which is associated with AD (11).
Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display increased affinity for poly-Ubiquitin moieties over the single Ubiquitin binding associated domain (UBA). TUBEs also display a protective effect on poly-ubiquitinated proteins, allowing for detection at relatively low abundance. This affinity resin can be used for the enrichment, isolation and identification of K48-linked (preferentially) or K63-linked poly-Ubiquitin chains or ubiquitinated substrates that contain these linkages.
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