Recombinant Human UbcH5c/UBE2D3 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human UbcH5c/UBE2D3 Protein, CF Summary

Details of Functionality
Recombinant Human UbcH5c/UBE2D3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5c/UBE2D3 concentration of 0.1-1 μM.
Source
E. coli-derived human UbcH5c/UBE2D3 protein
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBE2D3
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
E2-627 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UbcH5c/UBE2D3 Protein, CF

  • E2(17)KB 3
  • E2(17)KB3
  • EC 6.3.2.19
  • MGC43926
  • MGC5416
  • UBC4/5
  • UBC5C
  • UbcH5c
  • UBE2D3
  • Ubiquitin carrier protein D3
  • ubiquitin-conjugating enzyme E2 D3
  • Ubiquitin-conjugating enzyme E2(17)KB 3
  • Ubiquitin-conjugating enzyme E2-17 kDa 3
  • ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5)
  • ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
  • Ubiquitin-protein ligase D3

Background

Ubiquitin-conjugating Enzyme H5c (UbcH5c), also known as Ubiquitin-conjugating Enzyme E2D 3 (UBE2D3), is a member of the yeast Ubc4/5 family of Ubiquitin-conjugating (E2) enzymes. Human UbcH5c/UBE2D3 has a predicted molecular weight of 17 kDa and shares 88% and 89% amino acid sequence identity with the related family members, UbcH5a and UbcH5b, respectively. In combination with Ubiquitin ligases (E3s) such as CHIP, UbcH5c/UBE2D3 mediates the ubiquitination and subsequent degradation of several regulatory proteins (1). For instance, UbcH5c/UBE2D3 is involved in the poly-ubiquitination and proteasome-mediated degradation of the Nuclear Factor kappaB (NF-kappaB) inhibitor, IkappaB-alpha, and is implicated in NF-kappaB-dependent inflammation (2-4). UbcH5c/UBE2D3 also mediates the ubiquitination of Histone H2A and PCNA, suggesting that it functions during transcriptional regulation, DNA replication, and DNA damage responses (5-7).

  1. Page, R.C. et al. (2012) Biochemistry 51:4175.
  2. Gonen, H. et al. (1999) J. Biol. Chem. 274:14823.
  3. Shembade, N. et al. (2010) Science 327:1135.
  4. Xia, Z.P. et al. (2009) Nature 461:114.
  5. Zhang, S. et al. (2008) Cell Cycle 7:3399.
  6. Bentley, M.L. et al. (2011) EMBO J. 30:3285.
  7. Polanowska, J. et al. (2006) EMBO J. 25:2178. 

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Publications for UbcH5c/UBE2D3 (E2-627)(33)

We have publications tested in 4 confirmed species: Human, N/A, Plant - Medicago truncatula, Worm - Spodoptera frugiperda (Fall Army Worm).

We have publications tested in 3 applications: Bioassay, Enzyme Assay, Ubiquitination.


Filter By Application
Bioassay
(27)
Enzyme Assay
(1)
Ubiquitination
(3)
All Applications
Filter By Species
Human
(18)
N/A
(5)
Plant - Medicago truncatula
(1)
Worm - Spodoptera frugiperda (Fall Army Worm)
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using E2-627 Applications Species
P Wang, F Yan, Z Li, Y Yu, SE Parnell, Y Xiong Impaired plasma membrane localization of ubiquitin ligase complex underlies 3-M syndrome development J. Clin. Invest., 2019;130(0):. 2019 [PMID: 31343991] (Bioassay, Human) Bioassay Human
P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B Mao Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning Cell Rep, 2019;28(2):541-553.e4. 2019 [PMID: 31291587] (Bioassay) Bioassay
CM Xie, M Tan, XT Lin, D Wu, Y Jiang, Y Tan, H Li, Y Ma, X Xiong, Y Sun The FBXW7-SHOC2-Raptor Axis Controls the Cross-Talks between the RAS-ERK and mTORC1 Signaling Pathways Cell Rep, 2019;26(11):3037-3050.e4. 2019 [PMID: 30865892] (Bioassay) Bioassay
B Liu, J Yi, X Yang, L Liu, X Lou, Z Zhang, H Qi, Z Wang, J Zou, WG Zhu, W Gu, J Luo MDM2-mediated degradation of WRN promotes cellular senescence in a p53-independent manner Oncogene, 2018;0(0):. 2018 [PMID: 30532073] (Bioassay, Human) Bioassay Human
KA Donovan, J An, RP Nowak, JC Yuan, EC Fink, BC Berry, BL Ebert, ES Fischer Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome Elife, 2018;7(0):. 2018 [PMID: 30067223] (Ubiquitination, Human) Ubiquitination Human
M Yao, X Zhou, J Zhou, S Gong, G Hu, J Li, K Huang, P Lai, G Shi, AP Hutchins, H Sun, H Wang, H Yao PCGF5 is required for neural differentiation of embryonic stem cells Nat Commun, 2018;9(1):1463. 2018 [PMID: 29765032] (Bioassay, Human) Bioassay Human
J Liu, J Deng, F Zhu, Y Li, Z Lu, P Qin, T Wang, J Dong The MtDMI2-MtPUB2 negative feedback loop plays a role in nodulation homeostasis Plant Physiol., 2018;0(0):. 2018 [PMID: 29440269] (Bioassay, Plant - Medicago truncatula) Bioassay Plant - Medicago truncatula
SY Kim, HJ Kim, HJ Kim, DH Kim, CH Kim, JH Han, HK Byeon, K Lee HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer Autophagy, 2017;0(0):1-89. 2017 [PMID: 29260979] (Bioassay, Human) Bioassay Human
JR Brickner, JM Soll, PM Lombardi, CB Vågbø, MC Mudge, C Oyeniran, R Rabe, J Jackson, ME Sullender, E Blazosky, AK Byrum, Y Zhao, MA Corbett, J Gécz, M Field, A Vindigni, G Slupphaug, C Wolberger, N Mosammapar A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair Nature, 2017;551(7680):389-393. 2017 [PMID: 29144457] (Bioassay, Human) Bioassay Human
M Bosshard, R Aprigliano, C Gattiker, V Palibrk, E Markkanen, P Hoff Backe, S Pellegrino, FL Raymond, G Froyen, M Altmeyer, M Bjørås, GL Dianov, B van Loon Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability Sci Rep, 2017;7(1):15050. 2017 [PMID: 29118367] (Bioassay, Human) Bioassay Human
Show All 33 Publications.

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Bioinformatics

Gene Symbol UBE2D3
Uniprot