Recombinant Human SIRP gamma/CD172g Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human SIRP gamma/CD172g Fc Chimera Protein, CF Summary

Additional Information
Product is in the process of discontinuation
Details of Functionality
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 100-500 ng/mL anti-CD3 and recombinant human SIRP gamma.
The ED50 for this effect is 1-6 µg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human SIRP gamma/CD172g protein
Human SIRP gamma
(Val64-Ser364) (Val263Ala) (Ser286Leu)
Accession # Q9P1W8
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Val64
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
SIRPG
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
68-75 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SIRP gamma/CD172g Fc Chimera Protein, CF

  • bA77C3.1
  • CD172g antigen
  • CD172g
  • FLJ42230
  • signal-regulatory protein beta 2
  • Signal-regulatory protein beta-2
  • signal-regulatory protein gamma
  • SIRP beta 2
  • SIRP gamma
  • SIRP-B2
  • SIRPB2CD172 antigen-like family member B
  • SIRP-beta-2
  • SIRPG
  • SIRPgamma
  • SIRP-gamma

Background

Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily (1-5). SIRP members are “paired receptors” with homology in the extracellular domain but variability in the
|C‑terminus and signaling function (1, 2). The 387 amino acid (aa) SIRP gamma sequence contains a 28 aa potential signal sequence, a 332 aa extracellular domain (ECD) with four potential N‑glycosylation sites, a 23 aa transmembrane domain and a 4 aa cytoplasmic sequence. SIRP gamma contains one V-type Ig‑like domain that contains a J‑like sequence and two C1-type Ig‑like domains within its ECD (1, 2). Isoforms that lack one (isoform 2, 276 aa) or two (isoform 3, 170 aa) membrane-proximal
C‑type Ig-like domains have been described (5). Within the ECD, human SIRP gamma isoform 1 shares 78% aa identity with human SIRP beta 1, and appears to have structurally similar orthologs only in rhesus monkey and chimpanzee (100% and 91% aa identity, respectively) (2). SIRP gamma is the only SIRP known to be expressed on T cells, CD56bright NK cells and activated NK cells; it is not expressed on myeloid cells (5, 6). It shows adhesion to CD47, but at lower affinity than SIRP alpha (6). Expression of SIRP gamma on T cells suggests a role as an accessory protein interacting with CD47‑expressing antigen presenting cells (5, 6). Unlike SIRP alpha that has cytoplasmic ITIM domains, and SIRP beta 1 that interacts with DAP-12, SIRP gamma does not contain any obvious signaling mechanism (1, 2, 6). However, SIRP gamma -mediated adhesion appears to promote antigen-specific T cell proliferation and costimulate T cell activation (5).

  1. Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.
  2. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
  3. van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
  4. Ichigotani, Y. et al. (2000) J. Hum. Genet. 45:378.
  5. Piccio, L. et al. (2005) Blood 105:2421.
  6. Brooke, G. et al. (2004) J. Immunol. 173:2562.

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Bioinformatics

Gene Symbol SIRPG
Entrez
Uniprot