Recombinant Human Semaphorin 4A Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Semaphorin 4A Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 2.5-5.0 µg/mL of Recombinant Human Semaphorin 4A Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of Recombinant Human beta ‑NGF (Catalog # 256-GF).
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human Semaphorin 4A protein
Human Semaphorin 4A
(Gly32-His 683)
Accession # Q9H3S1
IRGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Gly32
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
SEMA4A
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
98.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
105-120 kDa, reducing conditions
Publications
Read Publications using
4694-S4 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 20.
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Semaphorin 4A Fc Chimera Protein, CF

  • CORD10
  • RP35
  • Sema B
  • sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4A
  • SEMA4A
  • SEMAB
  • Semaphorin 4A
  • semaphorin-4A
  • semaphorin-B
  • SEMB
  • SEMBFLJ12287

Background

Semaphorin 4A (Sema4A, previously semB) is a Class 4 transmembrane Semaphorin with activity in the immune and nervous systems (1). The 761 amino acid (aa) human Sema4A precursor contains a 32 aa signal sequence, a 651 aa extracellular domain (ECD) containing sema, PSI and C2-type immunoglobulin domains, a 21 aa transmembrane domain, and a 57 aa cytoplasmic domain with two Ser/Thr phosphorylation sites (2). Human Sema4A ECD shares 87%, 87%, 86% and 85% aa identity with mouse, rat, bovine and canine Sema4A, respectively, and shares 32-37% aa identity with other human Sema4 family members. Of six reported splice variants with 723, 629, 370, 321, 236 and 220 aa, five lack the N-terminus and/or portions of the sema domain, and three lack the transmembrane and cytoplasmic domains in the C-terminus (3). Sema4A was first described as a molecule that enhances T cell activation and interacts with TIM-2 (T cell immunoglobulin and mucin domain-2 (4). Mice with targeted disruption of Sema4A show defects in dendritic cell-mediated T cell priming and Th1 responses (5). Roles for Sema4A have also been identified in the brain, the endothelium and the eye. It mediates hippocampal neuron growth cone collapse in vitro through interaction of the sema domain with plexin-B1 (6). Interaction of Sema4A with endothelial cell plexin-D1 causes opposition to the angiogenic, proliferative, chemotactic and integrin-mediated adhesive actions of VEGF (7). The retina of Sema4A-/- mice shows severe degeneration, and mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans (8, 9).

  1. Kumanogoh, A. et al. (2003) J. Cell Sci. 116:3463.
  2. Swissprot Accession # Q9H3S1.
  3. Entrez Accession # CAI15528, CAI15529, CAI15531, CAI15532, CAI15533 and EAW52993.
  4. Kumanogoh, A. et al. (2002) Nature 419:629.
  5. Kumanogoh, A. et al. (2005) Immunity 22:305.
  6. Yukawa, K. et al. (2005) Int. J. Mol. Med. 16:115.
  7. Toyofuku, T. et al. (2007) EMBO J. 26:1373.
  8. Rice, D.S. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:2767.
  9. Abid, A. et al. (2007) J. Med. Genet. 43:378.

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Publications for Semaphorin 4A (4694-S4)(2)

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Bioinformatics

Gene Symbol SEMA4A
Entrez
Uniprot