Recombinant Human S5a/Angiocidin Agarose Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human S5a/Angiocidin Agarose Protein, CF Summary

Details of Functionality
S5a/Angiocidin Agarose is ideal for the enrichment of known S5a/Angiocidin-interacting proteins as well as the discovery of novel S5a/Angiocidin-interacting proteins.
Source
E. coli-derived
Accession #
Protein/Peptide Type
Affinity Matrices
Gene
PSMD4

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 3 months from date of receipt, 2 to 8 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Buffer
Supplied as a 50% slurry (v/v) in a solution of 20% Ethanol in deionized water

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human S5a/Angiocidin Agarose Protein, CF

  • Angiocidin
  • ASF
  • Macropain
  • Mcb1
  • PSMD4
  • pUB-R5
  • Rpn10
  • S5a

Background

S5a/Angiocidin, also known as Anti-secretory Factor (ASF), is classified under the gene PSMD4, but is often referred to by a different name depending on the context in which it is described. S5a and ASF have identical 377 amino acid (aa) sequences, while Angiocidin is described as having an additional Gly255Glu256Arg257 sequence in its C-Terminus (1-3). The human protein shares 96% and 99% aa sequence identity with its mouse and rat orthologs, respectively. Structurally, it contains an N-terminal von Willebrand Factor type A domain and two C-terminal Ubiquitin-interacting motifs (UIM). It acts as a Ubiquitin-binding protein where it is most commonly referred to as S5a or in yeast as Rpn10. It is part of the 19S regulatory subunit of the 26S Proteasome where its UIM recognizes poly-ubiquitinated proteins destined for degradation (4). As a part of the proteasome complex, it may also recognize the Ubiquitin-like modifier FAT10 (5). Free cytoplasmic forms also exist where its ubiquitination is catalyzed by a range of Ubiquitin E3 ligases from different classes. Therefore, experimentally S5a/Angiocidin may act as a useful substrate to monitor the activity of (E3) ligases, independent of their specific mechanisms of action (6). In cancer biology, where it is often referred to as Angiocidin, it is shown to slow tumor progression. It is found in the extracellular matrix of certain tumor subtypes, and it may act by suppressing angiogenesis or by directly inhibiting tumor cell growth (7,8). It also is found in several biological fluids where it is known primarily as ASF. It suppresses fluid secretion in response to enterotoxin and may act as an anti-inflammatory factor (9-11).

 

S5a/Angiocidin has a low affinity for mono-, di-, and tri-Ubiquitin but binds efficiently to tetrameric ubiquitin and has a preference for longer Ubiquitin polymers. This affinity resin can be used for the enrichment, isolation and identification of ubiquitinated proteins, 26S substrates or proteins that contain ubiquitin-like domains.

  1. Nilsson, L.A. et al. (1992) J. Parasitol. 78:1055.
  2. Young, P. et al. (1998) J. Biol. Chem. 273:5461.
  3. Zhou, J. et al. (2004) J. Cell. Biochem. 92:125.
  4. Walters, K.J. et al. (2002) Biochemistry 41:1767.
  5. Rani, N. et al. (2011) Nature Commun. 3:749.
  6. Kim, H.T. & A.L. Goldberg (2012) Methods Mol. Biol. 832:653.
  7. Sabherwal, Y. et al. (2006) Exp. Cell Res. 312:2443.
  8. Godek, J. et al. (2011) Exp. Mol. Pathol. 90:244.
  9. Davidson, T.S. & W.J. Hickey (2004) J. Leukoc. Biol. 76:835.
  10. Davidson, T.S. & W.J. Hickey (2004) Lab. Invest. 84:307.
  11. Ulgheri, C. et al. (2010) Nutr. Res. Rev. 23:300.    
  12.       

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Other Available Formats

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Bioinformatics

Gene Symbol PSMD4
Uniprot
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