Recombinant Human RNF114 Protein, CF

0.5 μg, 1 μg, and 2 μg per lane of Recombinant Human RNF114 (Catalog # E3-304) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 27 kDa.

Product Discontinued

Recombinant Human RNF114 Protein, CF Summary

• Source: E. coli-derived human RNF114 protein
• Accession #: Q9Y508
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 27 kDa

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 48 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human RNF114 Protein, CF

• PSORS12
• RNF114
• ZNF228
• ZNF313

Background

RING finger protein 114 (RNF114) is an E3 Ubiquitin Ligase containing both RING and C2HC type zinc finger domains.  RNF114 activates cell cycle progression and suppresses cellular senescence by ubiquitinating and destabilizing p21^WAF1, p27^KIP1 and p57^KIP2, three members of cyclin-dependent kinase inhibitors.  RNF114 expression is elevated at the late G1 phase and is crucial for the G1-to-S phase transition.  Nimbolide, a terpenoid small molecule derived from Neem trees, interferes with RNF114-dependent ubiquitination of p21, resulting in its rapid stabilization.  Nimbolide has been shown to impair proliferation of breast cancer cells and is being used as a basis for development of targeted protein degradation.  This recombinant protein is untagged.

1. Bodduluru L.N. et al. (2014) Tox. In Vitro  5: 1026
2. Han J. et al. (2013) Cell Death & Diff.  20: 1055
3. Spradlin J.N. et al. (2019) Nat. Chem. Biol.  15: 747

Bioinformatics

• Uniprot:
  • Q9Y508()