Recombinant Human NEDD8 Vinyl Sulfone Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

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Recombinant Human NEDD8 Vinyl Sulfone Protein, CF Summary

Details of Functionality
Add Recombinant Human NEDD8 Vinyl Sulfone to in vitro assays to inhibit NEDD8-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human NEDD8 Vinyl Sulfone concentration of 1-5 μM.
Source
E. coli-derived human NEDD8 protein
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
NEDD8
Purity
>98%, by HPLC

Applications/Dilutions

Publications
Read Publication using UL-802.

Packaging, Storage & Formulations

Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Purity
>98%, by HPLC

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NEDD8 Vinyl Sulfone Protein, CF

  • FLJ43224
  • MGC104393
  • MGC125896
  • MGC125897
  • NEDD8
  • NEDD-8
  • Neddylin
  • Neural precursor cell expressed developmentally down-regulated protein 8
  • neural precursor cell expressed, developmentally down-regulated 8
  • Rub1
  • Ubiquitin-like protein Nedd8

Background

Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Related to Ubiquitin 1 (Rub1), is a 6-8 kDa member of the Ubiquitin family of proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. Pro-NEDD8 contains one Ubiquitin-like domain (aa 1-65) and a 4-5 aa C-terminal propeptide (1,2). Cleavage of the propeptide exposes a C-terminal glycine residue that is used to generate a glycine-lysine intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from mouse, rat, and canine. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits (3). Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes (4). Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates (5). For example, transfer of NEDD8 to the Cullin scaffold activates members of the Cullin-RING Ligase (CRL) family of Ubiquitin ligases (E3) (6-8). CRL-induced ubiquitination is known to regulate proteins that are important for cell cycle progression and cell survival (9,10). Physiologically, NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies (11,12).

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Publications for NEDD8 (UL-802)(1)

We have publications tested in 1 confirmed species: Primate - Chlorocebus aethiops (African Green Monkey).


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Other Available Formats

Vinyl Sulfone UL-802

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Bioinformatics

Gene Symbol NEDD8
Uniprot