Recombinant Human NEDD8 E1 (APPBP1/UBA3) Protein, CF

Images

 
There are currently no images for NEDD8 Activating Enzyme (APPBP1/UBA3) (E-313).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human NEDD8 E1 (APPBP1/UBA3) Protein, CF Summary

Details of Functionality
Recombinant Human NEDD8 E1 (APP-BP1/UBA3) is a member of the NEDD8-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a NEDD8-conjugating (E2) enzyme and a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human NEDD8 E1 (APP-BP1/UBA3) concentration of 50-200 nM.
Source
E. coli-derived human NEDD8 Activating Enzyme (APPBP1/UBA3) protein
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
NAE1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW

60 kDa (APP-BP1)

52 kDa (UBA3)

.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
E-313 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in HEPES and NaCl.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NEDD8 E1 (APPBP1/UBA3) Protein, CF

  • A-116A10.1
  • amyloid beta precursor protein binding protein 1
  • amyloid beta precursor protein binding protein 1, 59kDa
  • Amyloid beta precursor protein-binding protein 1, 59 kDa
  • amyloid beta precursor protein-binding protein 1, 59kD
  • Amyloid protein-binding protein 1
  • APPBP1APP-BP1
  • NEDD8 Activating Enzyme (APPBP1/UBA3)
  • NEDD8 activating enzyme E1 subunit 1
  • NEDD8-activating enzyme E1 regulatory subunit
  • NEDD8-activating enzyme E1 subunit
  • protooncogene protein 1
  • Proto-oncogene protein 1
  • ula-1

Background

Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8) Activating Enzyme (APP-BP1/UBA3) is a heterodimeric NEDD8-activating (E1) enzyme with a predicted molecular weight of 112 kDa. It is responsible for the first step in the conjugation of NEDD8 to protein substrates. The NEDD8 Activating Enzyme heterodimer is composed of a regulatory subunit, Amyloid beta Precursor Protein Binding Protein 1 (APP-BP1), and a catalytic subunit, Ubiquitin-like Modifier Activating Enzyme 3 (UBA3). Human APP-BP1 is a 534 amino acid (aa) protein with a predicted molecular weight of 60 kDa that is expressed ubiquitously in fetal tissues and in the adult brain (1). APP-BP1 is required for UBA3 neddylation activity, regulates enzyme specificity, and is expressed as two isoforms, the full length protein and a second isoform with an alternate N-terminal, aa1-17, sequence (2). APP-BP1 has been shown to drive cell cycle progression, and its expression is increased in the hippocampus of Alzheimer's disease brains (3,4). Human UBA3 is a 463 aa protein with a predicted molecular weight of 52 kDa. It is ubiquitously expressed and shares high aa sequence identity with the C-terminal domain of human UBE1 (5). UBA3 contains an ATP-binding domain and an active site cysteine residue, Cys237 in humans, which are both common to E1 enzymes. Like APP-BP1, two isoforms of UBA3 have been identified in humans, the full length protein and a truncated isoform, which lacks aa 8-21. UBA3 is required for cell cycle progression and has been shown to downregulate steroid receptor activation (4,6). Neddylation and its associated enzymes have been implicated in the progression of Alzheimer's disease, via neddylation of APP, and cancer via post-translational modification of oncogenes (7,8).

  1. Chow, N. et al. (1996) J Biol Chem 271:11339.
  2. Walden, H. et al. (2003) Mol Cell 12:1427.
  3. Chen, Y. et al. (2003) J Cell Biol 163:27.
  4. Chen, Y. et al. (2000) J Biol Chem 275:8929.
  5. Gong, L. & E.T. Yeh (1999) J Biol Chem 274:12036.
  6. Fan, M. et al. (2002) Mol Endocrinol 16:315.
  7. Chen, Y. et al. (2012) J Cell Mol Med [Epub ahead of print]
  8. Soucy, T.A. et al. (2010) Genes Cancer 1:708.

Customers Who Viewed This Item Also Viewed...

NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-01863
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-37523
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
NBP2-49443
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56552
Species: Hu
Applications: WB
NB100-168
Species: Hu, Mu(-)
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-IC
NBP2-13075
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
NB120-1868
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr, IHC-P, IP
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP1-55035
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF4885
Species: Mu
Applications: WB, IP
MAB3765
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICC, ICFlow
NBP1-82454
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-74413
Species: Hu, Mu, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF1384
Species: Hu
Applications: WB, IHC, ICC
NBP2-41182
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP2-15840
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF9024
Species: Mu, Rt
Applications: WB, IHC
NBP2-47645
Species: Hu, Mu, Rt
Applications: Flow, IHC-P, PAGE, IF

Publications for NEDD8 Activating Enzyme (APPBP1/UBA3) (E-313)(4)

Reviews for NEDD8 Activating Enzyme (APPBP1/UBA3) (E-313) (0)

There are no reviews for NEDD8 Activating Enzyme (APPBP1/UBA3) (E-313). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for NEDD8 Activating Enzyme (APPBP1/UBA3) (E-313) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NEDD8 Activating Enzyme (APPBP1/UBA3) Products

Bioinformatics Tool for NEDD8 Activating Enzyme (APPBP1/UBA3) (E-313)

Discover related pathways, diseases and genes to NEDD8 Activating Enzyme (APPBP1/UBA3) (E-313). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NEDD8 Activating Enzyme (APPBP1/UBA3) (E-313)

Discover more about diseases related to NEDD8 Activating Enzyme (APPBP1/UBA3) (E-313).
 

Pathways for NEDD8 Activating Enzyme (APPBP1/UBA3) (E-313)

View related products by pathway.

Blogs on NEDD8 Activating Enzyme (APPBP1/UBA3)

There are no specific blogs for NEDD8 Activating Enzyme (APPBP1/UBA3), but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human NEDD8 E1 (APPBP1/UBA3) Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol NAE1
Entrez
Uniprot