Recombinant Human/Mouse/Rat/Canine/Equine BDNF Protein, CF


1 μg/lane of Recombinant Human BDNF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human BDNF (Catalog #248‑BD/CF) stimulates cell proliferation in the BaF mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL.

Product Details

Reactivity Hu, Mu, Rt, Ca, EqSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human/Mouse/Rat/Canine/Equine BDNF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL.
The specific activity of Recombinant Human/Mouse/Rat/Canine/Equine BDNF is approximately 1.3 x 103 units/μg, which is calibrated against recombinant human BDNF WHO Standard (NIBSC code: 96/534).
Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera (Catalog # 688-TK) is coated at 1 μg/mL, Recombinant Human BDNF binds with an apparent Kd <1 nM.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
13.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
13-14 kDa, reducing conditions
Read Publications using
248-BD/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat/Canine/Equine BDNF Protein, CF

  • Abrineurin
  • ANON2
  • BDNF
  • brain-derived neurotrophic factor
  • BULN2
  • MGC34632
  • neurotrophin


Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins (1, 2). All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor (3).

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Applications: Bioactivity

Publications for BDNF (248-BD/CF)(84)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, N/A, Porcine, Primate - Callitrix jacchus (Common Marmoset).

We have publications tested in 6 applications: Bioassay, ELISA Standard, In Vivo, Surface Plasmon Resonance, WB Standard, cell culture.

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Primate - Callitrix jacchus (Common Marmoset)
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Showing Publications 1 - 10 of 84. Show All 84 Publications.
Publications using 248-BD/CF Applications Species
K Attoff, A Gliga, J Lundqvist, U Norinder, A Forsby Whole genome microarray analysis of neural progenitor C17.2 cells during differentiation and validation of 30 neural mRNA biomarkers for estimation of developmental neurotoxicity PLoS ONE, 2017;12(12):e0190066. 2017 [PMID: 29261810] (Bioassay, Mouse) Bioassay Mouse
EA Newman, S Chukkapall, D Bashllari, TT Thomas, RA Van Noord, ER Lawlor, MJ Hoenerhoff, AW Opipari, VP Opipari Alternative NHEJ pathway proteins as components of MYCN oncogenic activity in human neural crest stem cell differentiation: implications for neuroblastoma initiation Cell Death Dis, 2017;8(12):3208. 2017 [PMID: 29238067] (Bioassay, Human) Bioassay Human
O Awad, LM Panicker, RM Deranieh, MP Srikanth, RA Brown, A Voit, T Peesay, TS Park, ET Zambidis, RA Feldman Altered Differentiation Potential of Gaucher&#039;s Disease iPSC Neuronal Progenitors due to Wnt/?-Catenin Downregulation Stem Cell Reports, 2017;9(6):1853-1867. 2017 [PMID: 29198828] (Bioassay, Human) Bioassay Human
T Yokoi, T Tanaka, E Matsuzaka, F Tamalu, SI Watanabe, S Nishina, N Azuma Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells Sci Rep, 2017;7(1):16757. 2017 [PMID: 29196712] (Bioassay, Human) Bioassay Human
KY Tseng, JE Anttila, K Khodosevic, RK Tuominen, M Lindahl, A Domanskyi, M Airavaara MANF Promotes Differentiation and Migration of Neural Progenitor Cells with Potential Neural Regenerative Effects in Stroke Mol. Ther., 2017;0(0):. 2017 [PMID: 29050872] (Bioassay, Mouse) Bioassay Mouse
W Wu, XL Wu, YQ Ji, Z Gao Differentiation of nestin?negative human hair follicle outer root sheath cells into neurons in�vitro Mol Med Rep, 2017;0(0):. 2017 [PMID: 28534946] (Bioassay, Human) Bioassay Human
K Burk, JD Murdoch, S Freytag, M Koenig, V Bharat, R Markworth, S Burkhardt, A Fischer, C Dean EndophilinAs regulate endosomal sorting of BDNF-TrkB to mediate survival signaling in hippocampal neurons Sci Rep, 2017;7(1):2149. 2017 [PMID: 28526875] (Bioassay, Mouse) Bioassay Mouse
H Kilpinen, A Goncalves, A Leha, V Afzal, K Alasoo, S Ashford, S Bala, D Bensaddek, FP Casale, OJ Culley, P Danecek, A Faulconbri, PW Harrison, A Kathuria, D McCarthy, SA McCarthy, R Meleckyte, Y Memari, N Moens, F Soares, A Mann, I Streeter, CA Agu, A Alderton, R Nelson, S Harper, M Patel, A White, SR Patel, L Clarke, R Halai, CM Kirton, A Kolb-Kokoc, P Beales, E Birney, D Danovi, AI Lamond, WH Ouwehand, L Vallier, FM Watt, R Durbin, O Stegle, DJ Gaffney Common genetic variation drives molecular heterogeneity in human iPSCs Nature, 2017;546(7658):370-375. 2017 [PMID: 28489815] (Bioassay, Human) Bioassay Human
X Yuan, Z Li, AC Baines, E Gavriilaki, Z Ye, Z Wen, EM Braunstein, LG Biesecker, L Cheng, X Dong, RA Brodsky A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model PLoS ONE, 2017;12(4):e0174074. 2017 [PMID: 28441409] (Bioassay, Human) Bioassay Human
L Guillaud, D Dimitrov, T Takahashi Presynaptic morphology and vesicular composition determine vesicle dynamics in mouse central synapses Elife, 2017;6(0):. 2017 [PMID: 28432787] (Bioassay, Human) Bioassay Human
Show All 84 Publications.

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The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

BDNF Antibodies and Synaptic Research
Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophins. During development it regulates the survival and differentiation of neuronal cell populations in the central and peripheral nervous system, while in adult synapse...  Read full blog post.

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Gene Symbol BDNF