Recombinant Human MD-1 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity

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Recombinant Human MD-1 Protein Summary

Details of Functionality
Measured by its ability to bind rmRP105/Fc Chimera in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived human MD-1 protein
Human MD-1
(Gly21 - Ser162)
Accession # O95711
IEGR GGSGGSGGS 10-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Gly21
Protein/Peptide Type
Recombinant Proteins
Gene
LY86
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
28 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MD-1 Protein

  • LY86
  • Ly-86
  • lymphocyte antigen 86
  • MD1
  • MD-1
  • MMD-1
  • Protein MD-1
  • RP105-associated

Background

MD-1 is a secreted glycoprotein that was originally identified as a v-myb-regulated gene from avian myeloleukemia virus-transformed chicken myeloblasts. MD-1 homologues were subsequently cloned from human and mouse. MD-1 was found to be a molecule that is associated with RP105, a type I transmembrane glycoprotein with extracellular leucine-rich repeats (LRR) typically found in Toll-like receptor (TLR) family members. However, RP105 has a short cytoplasmic tail and lacks the Toll-IL-1 R (TIR) domain that defines the IL-1 R/TLR superfamily (1 - 3). RP105 plays an important role in B-cell activation by bacterial lipopolysaccharide (LPS). It is expressed primarily on mature B cells, dendritic cells and macrophages (3).

Human MD-1 cDNA encodes a 162 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 38% and 66% amino acid sequence identity with chicken and mouse MD-1 respectively (1, 2). MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney. MD-1 is required for efficient RP105 cell surface expression and function (1 - 4). Cell surface RP105/MD-1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells. Activation of the RP105 complex has been shown to protect against apoptosis, induce B-cell proliferation and upregulate B7.2, a co-stimulatory molecule (4, 5). Since MD-1 is also expressed in liver and brain where RP105 is absent, it has been speculated that MD-1 can also be associated with other LRR-containing molecules, or have additional functions outside the immune system (5).

  1. Miyake, K. et al. (1998) J. Immun. 161:1348.
  2. Miura, Y. et al. (1998) Blood 92:2815.
  3. Miyake, K. et al. (1995) J. Immunol. 154:3333.
  4. Nagai, Y. et al. (2002) Blood 99:1699.
  5. Ogata, H. et al. (2000) J. Exp. Med. 192:23.

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Bioinformatics

Gene Symbol LY86
Uniprot