Recombinant Human IL-6 R alpha Protein, CF

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1 μg/lane of Recombinant Human IL-6 R alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 45‑50 kDa.
Recombinant Human IL-6 R alpha (Catalog # 227-SR/CF) enhances the IL-6 activity on the M1 mouse myeloid leukemia cell line. The ED50 for this effect is 5-15 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human IL-6 R alpha Protein, CF Summary

Details of Functionality
Measured by its ability to enhance the IL-6 activity on M1 mouse myeloid leukemia cells. Saito, T. et al. (1991) J. Immunol. 147:168. The ED50 for this effect is 5-15 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-6 R alpha protein
Leu20-Asp358
Accession #
N-terminal Sequence
Leu20
Protein/Peptide Type
Recombinant Proteins
Gene
IL6R
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
45-50 kDa, reducing conditions
Publications
Read Publications using
227-SR/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-6 R alpha Protein, CF

  • CD126
  • gp80
  • IL-6 R alpha
  • IL6Q
  • IL6R alpha
  • IL6R
  • IL-6R-1
  • IL6RA
  • IL-6Ra
  • IL6RQ
  • interleukin 6 receptor

Background

The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R and gp130 have been cloned, sequenced, and expressed (1 - 4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 alpha R cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

  1. Yamasaki et al. (1988) Science 241:825.
  2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
  3. Hibi et al. (1990) Cell 63:1149.
  4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
  5. Novick et al., (1989) J. Exp. Med. 170:1409.
  6. Yasukawa et al. (1990) J. Biochem. 108:673.
  7. Saito et al. (1991) J. Immunology 147:168.

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Publications for IL-6 R alpha (227-SR/CF)(28)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 6 applications: Bioassay, Blocking, ELISA Standard, EnzAct, In Vivo, cell culture.


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Bioassay
(20)
Blocking
(1)
ELISA Standard
(3)
EnzAct
(1)
In Vivo
(1)
cell culture
(2)
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Filter By Species
Human
(24)
Mouse
(1)
N/A
(3)
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Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using 227-SR/CF Applications Species
M Lyman, V Lieuw, R Richardson, A Timmer, C Stewart, S Granger, R Woods, M Silacci, D Grabulovsk, R Newman A bispecific antibody that targets IL-6 receptor and IL-17A for the potential therapy of patients with autoimmune and inflammatory diseases J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29678878] (Bioassay, Human) Bioassay Human
RL Huang, Y Sun, CK Ho, K Liu, QQ Tang, Y Xie, Q Li IL-6 potentiates BMP-2-induced osteogenesis and adipogenesis via two different BMPR1A-mediated pathways Cell Death Dis, 2018;9(2):144. 2018 [PMID: 29396550] (Bioassay, Human) Bioassay Human
AK Ravi, J Plumb, R Gaskell, S Mason, CS Broome, G Booth, M Catley, J Vestbo, D Singh COPD monocytes demonstrate impaired migratory ability Respir. Res., 2017;18(1):90. 2017 [PMID: 28494757] (Bioassay, Human) Bioassay Human
JV Larsen, CM Petersen SorLA in interleukin-6 signaling and turnover Mol. Cell. Biol, 2017;0(0):. 2017 [PMID: 28265003] (Bioassay, Human) Bioassay Human
Structure-based virtual screening and characterization of a novel IL-6 antagonistic compound from synthetic compound database Drug Des Devel Ther, 2016;10(0):4091-4100. 2016 [PMID: 28008232] (EnzAct, Human) EnzAct Human
STAT3-induced WDR1 overexpression promotes breast cancer cell migration Cell Signal, 2016;0(0):. 2016 [PMID: 27521604] (Bioassay, Human) Bioassay Human
Zhong H, Davis A, Ouzounova M, Carrasco R, Chen C, Breen S, Chang Y, Huang J, Liu Z, Yao Y, Hurt E, Moisan J, Fung M, Tice D, Clouthier S, Xiao Z, Wicha M, Korkaya H, Hollingsworth R A Novel IL6 Antibody Sensitizes Multiple Tumor Types to Chemotherapy Including Trastuzumab-Resistant Tumors. Cancer Res, 2016;76(2):480-90. 2016 [PMID: 26744529] (Bioassay, Human) Bioassay Human
Bhardwaj R, Yester J, Singh S, Biswas D, Surace M, Waters M, Hauser K, Yao Z, Boyce B, Kordula T RelB/p50 complexes regulate cytokine-induced YKL-40 expression. J Immunol, 2015;194(6):2862-70. 2015 [PMID: 25681350] (Bioassay, Human) Bioassay Human
Isobe, Aki, Sawada, Kenjiro, Kinose, Yasuto, Ohyagi-Hara, Chifumi, Nakatsuka, Erika, Makino, Hiroshi, Ogura, Tomonori, Mizuno, Tomoko, Suzuki, Noriko, Morii, Eiichi, Nakamura, Koji, Sawada, Ikuko, Toda, Aska, Hashimoto, Kae, Mabuchi, Seiji, Ohta, Tsuyoshi, Morishige, Ken-ichi, Kurachi, Hirohisa, Kimura, Tadashi Interleukin 6 receptor is an independent prognostic factor and a potential therapeutic target of ovarian cancer. PLoS ONE, 2015;10(2):e0118080. 2015 [PMID: 25658637] (Bioassay, Human) Bioassay Human
Sobotta M, Liou W, Stocker S, Talwar D, Oehler M, Ruppert T, Scharf A, Dick T Peroxiredoxin-2 and STAT3 form a redox relay for H2O2 signaling. Nat Chem Biol, 2015;11(1):64-70. 2015 [PMID: 25402766] (Bioassay, Human) Bioassay Human
Show All 28 Publications.

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Bioinformatics

Gene Symbol IL6R
Uniprot