Recombinant Human IGF-II R Protein, CF

Images

 
There are currently no images for IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) (2447-GR).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human IGF-II R Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rhIGF-II R at 2 µg/mL (100 µL/well) can bind rhIGF-II with a linear range of 2-100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IGF-II R protein
Ser1510-Phe2108, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ser1510
Protein/Peptide Type
Recombinant Proteins
Gene
IGF2R
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
80-83 kDa, reducing conditions
Publications
Read Publication using
2447-GR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGF-II R Protein, CF

  • 300 kDa mannose 6-phosphate receptor
  • cation-independent mannose-6 phosphate receptor
  • cation-independent mannose-6-phosphate receptor
  • CD222 antigen
  • CD222
  • CI Man-6-P receptor
  • CIMPR
  • CI-MPR
  • IGF2R
  • IGF-II R
  • IGF-II receptor
  • IGFIIR
  • IGF-IIR
  • insulin-like growth factor 2 receptorM6P/IGF2R
  • Insulin-like growth factor II receptor
  • M6P/IGF2 receptor
  • M6PR
  • M6P-R
  • MPR 300
  • MPR1
  • MPRI
  • MPRIM6PR

Background

The type 2 insulin-like growth factor receptor (also known as cation-independent mannose-6 phosphate receptor/CI-MPR) is a 300 kDa member of the P-type lectin family of molecules. P-type lectins generate functional eukaryotic lysosomes by binding and sorting lysosomal enzymes expressing phosphorylated mannose residues (M6P) (1-3). IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment and a 124 aa cytoplasmic tail (4, 5). The extracellular region consists of 15 contiguous “binding” repeats of about 150 aa each. The odd-numbered repeats interact with “ligands” while the even-numbered repeats likely generate a nondisulfide homodimer in the membrane (1). Repeat #11 binds IGF-II, while repeats 3 and 9 bind mannose-6 phosphate; repeat #13 contains a fibronectin type II motif and assists in IGF-II binding (1, 2). In the extracellular region of IGF-II R expressed by R&D Systems (600 aa’s), human IGF-II R is 85% aa identical to both mouse and bovine IGF-II R. This expressed region includes binding repeats #11, 12, and 13. In addition to IGF-II, CI-MPR/IGF-II R binds non-M6P containing ligands such as retinoic acid, urokinase-type plasminogen-activator receptor and plasminogen, plus
M6P-containing molecules such as lysosomal enzymes, TGF-beta 1 precursor, proliferin, LIF, CD26, herpes simplex glycoprotein D and granzymes A and B (2, 6). IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P-tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, it would appear that IGF-II R is principally a regulator of local IGF-II levels, targeting IGF-II for destruction in lysosomes (2). However, some evidence suggests the receptor will signal via G-proteins, an effect that has yet to be conclusively shown (6).

  1. Ghosh, P. et al. (2003) Nat. Rev. Mol. Cell. Biol. 4:202.
  2. Dahms, N.M. and M.K. Hancock (2002) Biochim. Biophys. Acta. 1572:317.
  3. Zaina, S. and J. Nilsson (2003) Curr. Opin. Lipidol. 14:483.
  4. Morgan, D.O. et al. (1987) Nature 329:301.
  5. Oshima, A. et al. (1988) J. Biol. Chem. 263:2553.
  6. Hawkes, C. and S. Kar (2004) Brain Res. Rev. 44:117.

Customers Who Viewed This Item Also Viewed...

MAB391
Species: Hu, Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP2-12793
Species: Hu, Mu
Applications: WB, IP
AF1029
Species: Mu
Applications: WB, IHC, IP
NBP2-20439
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-44050
Species: Mu, Rt
Applications: WB, PEP-ELISA
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
AF1310
Species: Hu
Applications: WB, IHC, IP
NBP1-28566
Species: Hu, Mu, Rt, Bv, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
NBP1-89917
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-83220
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
2447-GR
Species: Hu
Applications: Binding Activity

Publications for IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) (2447-GR)(1)

We have publications tested in 1 confirmed species: N/A.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(1)
All Applications
Filter By Species
N/A
(1)
All Species

Reviews for IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) (2447-GR) (0)

There are no reviews for IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) (2447-GR). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) (2447-GR) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) (2447-GR)

Discover related pathways, diseases and genes to IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) (2447-GR). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) (2447-GR)

Discover more about diseases related to IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) (2447-GR).
 

Pathways for IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) (2447-GR)

View related products by pathway.

PTMs for IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) (2447-GR)

Learn more about PTMs related to IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) (2447-GR).

Blogs on IGF-II R/Mannose 6 Phosphate Receptor (Cation independent)

There are no specific blogs for IGF-II R/Mannose 6 Phosphate Receptor (Cation independent), but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human IGF-II R Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol IGF2R
Entrez
Uniprot