Recombinant Human IGF-II Protein, CF

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1 μg/lane of Recombinant Human IGF-II was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human IGF-II (Catalog # 292‑G2) stimulates cell proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 1.5‑6 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human IGF-II Protein, CF Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 1.5‑6 ng/mL.
Source
E. coli-derived human IGF-II protein
Ala25-Glu91
Accession #
N-terminal Sequence
Ala25
Protein/Peptide Type
Recombinant Proteins
Gene
IGF2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
7.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
292-G2 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetic Acid.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGF-II Protein, CF

  • C11orf43
  • chromosome 11 open reading frame 43
  • FLJ22066
  • FLJ44734
  • IGF2
  • IGF-2
  • IGFII
  • IGF-II
  • insulin-like growth factor 2 (somatomedin A)
  • insulin-like growth factor II
  • insulin-like growth factor type 2
  • MSA
  • PP9974
  • somatomedin-A

Background

Insulin-like growth factor I (also known as somatomedin C and somatomedin A) and insulin-like growth factor II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved (100% identity between human, bovine and porcine proteins) and exhibit cross-species activity.

IGF-II is a potent mitogenic growth factor. However, unlike IGF-I which has important postnatal roles, the growth-promoting function of IGF-II is limited to embryonic development.

Two specific cell surface receptors that bind IGF-I and IGF-II have been identified. The type I IGF receptor that participates in IGF signaling is structurally related to the insulin receptor. It is a disulfide-linked heterotetrameric transmembrane glycoprotein with an intracellular tyrosine kinase domain. Type I IGF receptor binds IGF-I with higher affinity than IGF-II. The type II IGF receptor which binds IGF-II with much higher affinity than IGF-I is also the cation-independent mannose 6-phosphate receptor. At the present time, it is not known if the type II IGF receptor participates in the IGF signaling pathway. An additional unknown receptor which mediates IGF‑II signaling has also been proposed. Circulating IGFs exist in complexes bound to IGF binding proteins. Currently, at least six high affinity binding proteins have been identified.

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Publications for IGF-II (292-G2)(32)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bovine, Chicken, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque).

We have publications tested in 5 applications: Bioassay, ELISA Standard, Enzyme Substrate, In Vivo, Library Screening.


Filter By Application
Bioassay
(24)
ELISA Standard
(3)
Enzyme Substrate
(1)
In Vivo
(2)
Library Screening
(1)
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Filter By Species
Human
(22)
Mouse
(1)
Rat
(1)
Bovine
(1)
Chicken
(1)
N/A
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
(1)
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using 292-G2 Applications Species
Z Chen, J Liu, D Chu, Y Shan, G Ma, H Zhang, XD Zhang, P Wang, Q Chen, C Deng, W Chen, DS Dimitrov, Q Zhao A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells Int. J. Biol. Sci., 2018;14(7):799-806. 2018 [PMID: 29910690] (Library Screening, Human) Library Screening Human
R Sambathkum, R Akkerman, S Dastidar, P Roelandt, M Kumar, M Bajaj, AR Mestre Ros, N Helsen, V Vanslembro, E Kalo, S Khurana, J Laureys, C Gysemans, MM Faas, P de Vos, CM Verfaillie Generation of hepatocyte- and endocrine pancreatic-like cells from human induced endodermal progenitor cells PLoS ONE, 2018;13(5):e0197046. 2018 [PMID: 29750821] (Bioassay, Human) Bioassay Human
R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29339495] (Bioassay) Bioassay
SP Guan, ATL Lam, JP Newman, KLM Chua, CYL Kok, ST Chong, MLK Chua, PYP Lam Matrix metalloproteinase-1 facilitates MSC migration via cleavage of IGF-2/IGFBP2 complex FEBS Open Bio, 2018;8(1):15-26. 2018 [PMID: 29321953] (Bioassay, Human) Bioassay Human
Y Liu, C Yu, Y Wu, X Sun, Q Su, C You, H Xin CD44(+) fibroblasts increases breast cancer cell survival and drug resistance via IGF2BP3-CD44-IGF2 signalling J. Cell. Mol. Med., 2017;0(0):. 2017 [PMID: 28523716] (ELISA Standard, Human) ELISA Standard Human
Amber N Stratman Mural-Endothelial cell-cell interactions stabilize the developing zebrafish dorsal aorta Development, 2016;0(0):. 2016 [PMID: 27913637] (Bioassay, Human) Bioassay Human
Tong Wu TGF? signaling confers sorafenib resistance via induction of multiple RTKs in hepatocellular carcinoma cells Mol. Carcinog., 2016;0(0):. 2016 [PMID: 27862334] (Bioassay, Human) Bioassay Human
Addiction to the IGF2-ID1-IGF2 circuit for maintenance of the breast cancer stem-like cells Oncogene, 2016;0(0):. 2016 [PMID: 27546618] (Bioassay, Human) Bioassay Human
G Cassinelli, E Favini, L Dal Bo, M Tortoreto, M De Maglie, G Dagrada, S Pilotti, F Zunino, N Zaffaroni, C Lanzi Antitumor efficacy of the heparan sulfate mimic roneparstat (SST0001) against sarcoma models involves multi-target inhibition of receptor tyrosine kinases Oncotarget, 2016;7(30):47848-47863. 2016 [PMID: 27374103]
Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS Sci Rep, 2016;6(0):25960. 2016 [PMID: 27180807] (Bioassay, Human) Bioassay Human
Show All 32 Publications.

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Bioinformatics

Gene Symbol IGF2
Entrez
Uniprot