Recombinant Human IGF-I Protein, CF

Images

 
1 μg/lane of Recombinant Human IGF-I was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human IGF-I (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5 ng/mL.
MALDI-TOF analysis of Recombinant Human IGF-I.The major peak corresponds to the calculated molecular mass, 7650 Da. The minor peak at 7873 is a matrix-associated artifact of the MALDI-TOF.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human IGF-I Protein, CF Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.3-1.5 ng/mL.
The specific activity of recombinant human IGF-l is approximately 2.5 IU/μg, which is calibrated against recombinant human IGF-l WHO International Standard (NIBSC code: 91/554).
Source
E. coli-derived human IGF-I protein
Gly49-Ala118
Accession #
N-terminal Sequence
Gly49
Protein/Peptide Type
Recombinant Proteins
Gene
IGF1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
7 kDa, reducing conditions
Publications
Read Publications using
291-G1 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGF-I Protein, CF

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C

Background

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C‑terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF‑I is identical between isoforms and is generated by proteolytic removal of the N- and C‑terminal regions. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF‑II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4‑7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half‑life and modulates its interactions with receptors (10).

  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Sandberg-Nordqvist, A.C. et al. (1992) Brain Res. Mol. Brain Res. 12:275.
  3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
  9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

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291-G1
Species: Hu
Applications: Bioactivity

Publications for IGF-I (291-G1)(108)

We have publications tested in 11 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Insect, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque), Rabbit.

We have publications tested in 8 applications: Bioassay, BioassayCell, ELISA Standard, In Vivo, Neut, Scaffold Fabrication, WB Ctrl, cell culture.


Filter By Application
Bioassay
(83)
BioassayCell
(1)
ELISA Standard
(1)
In Vivo
(13)
Neut
(1)
Scaffold Fabrication
(1)
WB Ctrl
(1)
cell culture
(4)
All Applications
Filter By Species
Human
(72)
Mouse
(9)
Rat
(4)
Bovine
(7)
Canine
(2)
Chicken
(4)
Insect
(1)
N/A
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(2)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
(1)
Rabbit
(2)
All Species
Showing Publications 1 - 10 of 108. Show All 108 Publications.
Publications using 291-G1 Applications Species
LC Reis, EM Ramos-Sanc, F Petitto-As, AH Nerland, M Hernandez-, F Selheim, LM Floeter-Wi, H Goto Insulin-Like Growth Factor-I as an Effector Element of the Cytokine IL-4 in the Development of a Leishmania major Infection Mediators Inflamm., 2018;2018(0):9787128. 2018 [PMID: 30150896] (Mouse) Mouse
YY Lin, Y Chien, JH Chuang, CC Chang, YP Yang, YH Lai, WL Lo, KH Chien, TI Huo, CY Wang Development of a Graphene Oxide-Incorporated Polydimethylsiloxane Membrane with Hexagonal Micropillars Int J Mol Sci, 2018;19(9):. 2018 [PMID: 30149618] (Bioassay, Human) Bioassay Human
S Koyuncu, I Saez, HJ Lee, R Gutierrez-, W Pokrzywa, A Fatima, T Hoppe, D Vilchez The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington&#039;s disease patients Nat Commun, 2018;9(1):2886. 2018 [PMID: 30038412] (Bioassay, Human) Bioassay Human
FC Yang, J Draper, PG Smith, JL Vivian, SM Shapiro, JA Stanford Short Term Development and Fate of MGE-Like Neural Progenitor Cells in Jaundiced and Non-Jaundiced Rat Brain Cell Transplant, 2018;0(0):9636897187663. 2018 [PMID: 29845869] (Bioassay, Human) Bioassay Human
MK Shanmugam, KS Ahn, JH Lee, R Kannaiyan, N Mustafa, KA Manu, KS Siveen, G Sethi, WJ Chng, AP Kumar Celastrol Attenuates the Invasion and Migration and Augments the Anticancer Effects of Bortezomib in a Xenograft Mouse Model of Multiple Myeloma Front Pharmacol, 2018;9(0):365. 2018 [PMID: 29773987] (Bioassay, Human) Bioassay Human
S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma Oncotarget, 2018;9(18):14338-14353. 2018 [PMID: 29581848] (Bioassay, Human) Bioassay Human
G Zhou, H Jiang, Z Yin, Y Liu, Q Zhang, C Zhang, B Pan, J Zhou, X Zhou, H Sun, D Li, A He, Z Zhang, W Zhang, W Liu, Y Cao In Vitro Regeneration of Patient-specific Ear-shaped Cartilage and Its First Clinical Application for Auricular Reconstruction EBioMedicine, 2018;28(0):287-302. 2018 [PMID: 29396297] (Bioassay, Human) Bioassay Human
L Todd, L Suarez, C Quinn, AJ Fischer Retinoic Acid-Signaling Regulates the Proliferative and Neurogenic Capacity of M�ller Glia-Derived Progenitor Cells in the Avian Retina Stem Cells, 2017;0(0):. 2017 [PMID: 29193451] (In Vivo, Chicken) In Vivo Chicken
E Varga, C Nemes, I Bock, Z Táncos, S Berzsenyi, G Lévay, V Román, J Kobolák, A Dinnyés Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD) Stem Cell Res, 2017;21(0):19-22. 2017 [PMID: 28677532] (Bioassay, Human) Bioassay Human
P Haynes, K Kernan, SL Zhou, DG Miller Expression patterns of FSHD-causing DUX4 and myogenic transcription factors PAX3 and PAX7 are spatially distinct in differentiating human stem cell cultures Skelet Muscle, 2017;7(1):13. 2017 [PMID: 28637492] (Bioassay, Human) Bioassay Human
Show All 108 Publications.

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Bioinformatics

Gene Symbol IGF1
Entrez
Uniprot