Recombinant Human His6-USP22 Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human His6-USP22 Protein, CF Summary

Details of Functionality
Recombinant human USP22 is a Ubiquitin-specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial USP22 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) substrates.  Note: Recombinant USP22 is not active toward poly-Ubiquitin chains, Ubiquitinated histones and other Ubiquitinated protein substrates in the absence of adapter proteins such as ATXN7L or ENY2.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP22 protein
Contains an N-terminal 6-His tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.


Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, and 2 mM TCEP
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-USP22 Protein, CF

  • Deubiquitinating enzyme 22
  • EC
  • EC
  • KIAA1063ubiquitin carboxyl-terminal hydrolase 22
  • KIAA1064
  • ubiquitin specific peptidase 22
  • ubiquitin specific peptidase 3-like
  • ubiquitin specific protease 22
  • Ubiquitin Thioesterase 22
  • Ubiquitin thiolesterase 22
  • ubiquitin-specific processing protease 22
  • Ubiquitin-specific-processing protease 22
  • USP22
  • USP3L


Ubiquitin carboxyl-terminal hydrolase 22 (USP22) is a specialized cysteine protease with a predicted molecular weight of 60 kDa. USP22 is a member of the peptidase C19 family (UBP8 subfamily) and the human protein shares 98% amino acid sequence identity with its mouse ortholog. USP22 is a member of the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex, a 2 MDa protein machine that mediates the acetylation and deubiquitination of histones as well as non-histone substrates. Within SAGA, USP22 binds directly with ATXN7L3 and provides the activity required to deubiquitinate mono-Ubiquitinated histone H2B. Recent findings suggest USP27X and USP51, which function independently of SAGA, may compete with USP22 for ATXN7L3 and ENY2 binding, and that imbalances in these activities may contribute to human diseases including cancer.
  1. Atanassov B.S. et al. (2016) Mol. Cell 62:558.
  2.  Atanassov B.S. et al. (2011) FEBS Lett. 585:2016.
  3. Atanassov B.S. & Dent S.R. (2016) personal communication.
  4. Koutelou E. et al. (2010) Curr Opin Cell Biol. 22:374.
  5. Lang G. et al. (2011) Mol Cell Biol. 31:3734.
  6.  Zhang X.Y. et al. (2008) Mol. Cell 29:102.
  7. Zhao Y. et al. (2008) Mol. Cell 29:92.

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