Recombinant Human His6-USP13 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human His6-USP13 Protein, CF Summary

Details of Functionality

Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP13 concentration of 100-500 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP13 protein
Contains an C-terminal 6-His tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
USP13
Purity
>98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
98 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
E-588 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Purity
>98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-USP13 Protein, CF

  • Deubiquitinating enzyme 13
  • EC 3.1.2.15
  • EC 3.4.19.12
  • Isopeptidase T-3
  • IsoT-3
  • ISOT3IsoT-3
  • ubiquitin carboxyl-terminal hydrolase 13
  • ubiquitin specific peptidase 13 (isopeptidase T-3)
  • ubiquitin specific protease 13 (isopeptidase T-3)
  • Ubiquitin Thioesterase 13
  • Ubiquitin thiolesterase 13
  • Ubiquitin-specific-processing protease 13
  • USP13

Background

Ubiquitin carboxyl-terminal hydrolase 13 (USP13) is a specialized cysteine protease with a predicted molecular weight of 97 kDa. USP13 is a member of the peptidase C19 family and the human protein shares 96% amino acid sequence identity with its mouse ortholog. Recombinant USP13 has weak deubiquitinase activity against unanchored polyubiquitin chains, but will disassemble tetraubiquitin chains in extended reactions, particularly K63 and K48 linkages.  Suggested biological targets of USP13 include PTEN, BECN1, MITF, SKP2 and USP10, and roles for USP13 in melanoma and colorectal cancer have been reported. This recombinant protein contains a C-terminal 6-His tag.

  1. Xiang, S. et al. (2015) Oncol Rep. 33: 1342
  2. Zhang, J. et al. (2013) Nat Cell Biol. 15: 1486
  3. Zhang, Y-H. et al. (2011) PLoS One 6: e29362
  4. Zhao, X. et al. (2011) Nat Commun. 2: 414. doi: 10.1038/ncomms1421

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Publications for USP13 (E-588)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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(1)
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(1)
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Bioinformatics

Gene Symbol USP13
Entrez
Uniprot