Recombinant Human His6-Uev1a (Mms2)/UBE2V1 Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

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Recombinant Human His6-Uev1a (Mms2)/UBE2V1 Protein, CF Summary

Details of Functionality
Recombinant Human His6-Uev1a (Mms2)/UBE2V1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Uev1a (Mms2)/UBE2V1 concentration of 0.1-1 μM.
Source
E. coli-derived human Uev1a/UBE2V1 protein
Contains a 6-His tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBE2V1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in 50 mM Hepes pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 2 mM DTT
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-Uev1a (Mms2)/UBE2V1 Protein, CF

  • CIR1
  • CROC1
  • CROC1TRAF6-regulated IKK activator 1 beta Uev1A
  • CROC-1UBE2V
  • UBE2V1
  • ubiquitin-conjugating enzyme E2 variant 1
  • Uev1a
  • UEV-1CIR1
  • UEV1DNA-binding protein

Background

Ubiquitin-conjugating Enzyme Variant 1a (Uev1a), also known as Ubiquitin-conjugating Enzyme E2 Variant 1 (UBE2V1), is a member of the Ubiquitin-conjugating (E2) enzyme family. Human Uev1a/UBE2V1 and its mouse ortholog have predicted molecular weights of 19 kDa and 16.5 kDa, respectively. The human protein shares 89% amino acid (aa) sequence identity with its mouse ortholog. The E2 catalytic core domain of Uev1a/UBE2V1 lacks an active site cysteine residue, rendering it catalytically inactive on its own (1). However, in the cytoplasm Uev1a/UBE2V1 is able to form a catalytically active complex with UBE2N/Ubc13, which mediates the synthesis Lys63-linked Ubiquitin chains and is required for NF-kappa B activation (2,3). Uev1a/UBE2V1 is required for UBE2N (Ubc13)/Uev1a Complex-dependent Lys63-linked Ubiquitin chain formation. More specifically, Uev1a/UBE2V1 orients the Ubiquitin molecule to favor linkage at Lys63 via a non-covalent interaction with the Ubiquitin molecule (4). The human protein contains a non-conserved 30 aa N-terminal tail that functionally differentiates it from Mms2, another E2 enzyme variant and UBE2N/Ubc13 binding partner (4). Pharmacological inhibition of the UBE2N (Ubc13)/Uev1a Complex blocks the proliferation of diffuse large B-cell lymphoma cells, suggesting that this complex may play a role in cancer (5). This protein has an N-terminal His6-tag.

  1. Broomfield, S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5678.
  2. Andersen, P.L. et al. (2005) J. Cell Biol. 170:745.
  3. Pertel, T. et al. (2011) Nature 472:361.
  4. McKenna, S. et al. (2003) J. Biol. Chem. 278:13151.
  5. Pulvino, M. et al. (2012) Blood 120:1668.

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Bioinformatics

Gene Symbol UBE2V1
Uniprot