Recombinant Human His6-UBE2R2 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

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Recombinant Human His6-UBE2R2 Protein, CF Summary

Details of Functionality
Recombinant Human His6-UBE2R2 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2R2 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2R2 protein
Contains an N-terminal 6-His tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBE2R2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
E2-715 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-UBE2R2 Protein, CF

  • CDC34B
  • CDC34BEC 6.3.2.19
  • E2-CDC34B
  • FLJ20419
  • MGC10481
  • UBC3B
  • UBC3BUbiquitin-protein ligase R2
  • UBE2R2
  • Ubiquitin carrier protein R2
  • ubiquitin-conjugating enzyme E2 R2
  • Ubiquitin-conjugating enzyme E2-CDC34B
  • ubiquitin-conjugating enzyme E2R 2
  • ubiquitin-conjugating enzyme UBC3B

Background

Ubiquitin-conjugating Enzyme E2R 2 (UBE2R2), also known as CDC34B and UBC3B, is a 238 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of 27 kDa. Human UBE2R2 shares 100% aa sequence identity with its mouse ortholog and 78% aa sequence identity with its paralog, UBCH3. UBE2Q2 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). The UBE1 Ubiquitin-activating (E1) enzyme, but not the UBA6 E1 enzyme, is capable of charging UBE2R2 with Ubiquitin (2). UBE2R2 can be phosphorylated on Ser233 by Casein Kinase 2, which promotes an interaction with the beta -TrCP Ubiquitin ligase (E3) and the subsequent ubiquitination and degradation of beta -Catenin (1). This protein contains an N-terminal 6-His tag.

  1. Semplici, F. et al. (2002) Oncogene 21:3978.
  2. Jin, J. et al. (2007) Nature 447:1135.

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Publications for UBE2R2 (E2-715)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol UBE2R2
Uniprot