Recombinant Human His6-UBE2G1 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human His6-UBE2G1 Protein, CF Summary

Details of Functionality
Recombinant Human His6-UBE2G1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2G1 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2G1 protein
Contains an N-terminal 6-His tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBE2G1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
E2-700 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-UBE2G1 Protein, CF

  • E217K
  • EC 6.3.2.19
  • UBC7
  • UBE2G1
  • UBE2GE217K
  • Ubiquitin carrier protein G1
  • ubiquitin-conjugating enzyme E2 G1
  • ubiquitin-conjugating enzyme E2G 1 (homologous to C. elegans UBC7)
  • ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans)
  • ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast)
  • Ubiquitin-protein ligase G1

Background

Ubiquitin-conjugating Enzyme E2G 1 (UBE2G1) is a 170 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of 19.5 kDa. The human protein shares 100% aa sequence identity with the mouse and rat orthologs.  UBE2G1 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). The expression level of UBE2G1 is downregulated when miR-199a is overexpressed, suggesting that UBE2G1 mRNA is targeted by miR-199a (2). UBE2G1 has been reported to function with the CRL4(Cdt2) Ubiquitin ligase (E3) to promote the poly-ubiquitination and degradation of Cdt1 (3). Reduced expression of UBE2G1 in medulloblastoma tumors has also been reported, suggesting that it may act as a tumor suppressor (4). This protein contains an N-terminal 6-His tag.

  1. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  2. Haghikia, A. et al. (2011) Eur. Heart J. 32:1287.
  3. Shibata, E. et al. (2011) Mol. Cell. Biol. 31:3136.
  4. Cvekl, A. Jr. et al. (2004) Eur. J. Cancer 40:2525.

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Publications for UBE2G1 (E2-700)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, Ubiquitination.


Filter By Application
Bioassay
(1)
Ubiquitination
(1)
All Applications
Filter By Species
Human
(2)
All Species

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Bioinformatics

Gene Symbol UBE2G1
Uniprot