Recombinant Human His6-UBE2G1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2G1 concentration of 0.1-1 μM.
E. coli-derived human UBE2G1 protein Contains an N-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Ubiquitin-conjugating Enzyme E2G 1 (UBE2G1) is a 170 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of 19.5 kDa. The human protein shares 100% aa sequence identity with the mouse and rat orthologs. UBE2G1 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). The expression level of UBE2G1 is downregulated when miR-199a is overexpressed, suggesting that UBE2G1 mRNA is targeted by miR-199a (2). UBE2G1 has been reported to function with the CRL4(Cdt2) Ubiquitin ligase (E3) to promote the poly-ubiquitination and degradation of Cdt1 (3). Reduced expression of UBE2G1 in medulloblastoma tumors has also been reported, suggesting that it may act as a tumor suppressor (4). This protein contains an N-terminal 6-His tag.
Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
Haghikia, A. et al. (2011) Eur. Heart J. 32:1287.
Shibata, E. et al. (2011) Mol. Cell. Biol. 31:3136.
Cvekl, A. Jr. et al. (2004) Eur. J. Cancer 40:2525.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Recombinant Human His6-UBE2G1 Protein, CF and receive a gift card or discount.