Recombinant Human His6-UAF1 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human His6-UAF1 Protein, CF Summary

Details of Functionality

Recombinant Human UAF1 is an activator of Ubiquitin-specific deconjugating enzymes USP1, USP12, and USP46.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial Recombinant Human UAF1 concentration equal to the concentration of deconjugating enzyme present (1:1 stoichiometry) in in vitro deconjugation reactions utilizing Ubiquitin-AMC.

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human UAF1 protein
Contains an C-terminal 6-His tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
WDR48
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
E-566 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer

X mg/ml (X μM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-UAF1 Protein, CF

  • KIAA1449DKFZp686G1794
  • P80
  • UAF1
  • USP1 associated factor 1
  • USP1-associated factor 1
  • WD repeat domain 48
  • WD repeat endosomal protein
  • WD repeat-containing protein 48
  • WDR48

Background

UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) is a positive regulator of at least three deubiquitinating enzymes of the C19 peptidase family including USP1, USP12, and USP46. In the absence of UAF1, all three of these deubiquitinases are almost completely inactive, and available biochemical evidence suggests UAF1 activation occurs by increasing catalytic turnover as opposed to altered substrate affinity (at least in the case of UAF1/USP1) of the enzymes. Most notably, UAF1 strongly activates USP1 deubiquitination of monoubiquitinated FANCD2 and PCNA, thereby playing important roles in DNA damage response and translesion synthesis. The UAF1/USP12 complex has recently been described as a negative regulator of Notch signaling. Disruption of UAF1-dependent USP12 activity interrupts Notch trafficking to the lysosomes, leading to an increased amount of receptor at the cell surface and to higher Notch activity. At the biochemical level, UAF1/USP12 deubiquitinates the non-activated form of Notch in cell culture and in vitro. This recombinant protein contains a C-terminal 6-His tag.

  1. Cohn M.A., et al. (2009) J Biol. Chem. 284: 5343-5351
  2. Huang T.T., et al. (2006) Nat. Cell Biol. 8: 339-347
  3. Joo H-Y., et al. (2011) Biol. Chem. 286: 7190-7201
  4. Moretti J., et al. (2012) J Biol. Chem. 287: 29429-29441
  5. Williams S.A, et al. (2011) Cell 146: 918–930

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E-566
Species: Hu
Applications: Bioactivity

Publications for UAF1/WDR48 (E-566)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Ubiquitination.


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Ubiquitination
(1)
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Human
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Bioinformatics

Gene Symbol WDR48
Uniprot