Recombinant Human His6-NEDP1/SENP8 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human His6-NEDP1/SENP8 Protein, CF Summary

Details of Functionality
Recombinant Human His6-NEDP1/SENP8 is a NEDD8-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-NEDP1/SENP8 concentration of 50-500 nM. A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity.
Source
E. coli-derived human NEDP1/SENP8 protein
Contains a 6-His tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
SENP8
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 2 mM TCEP

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-NEDP1/SENP8 Protein, CF

  • DEN1
  • DEN1deneddylase-1
  • deneddylase 1
  • Deneddylase-1
  • EC 3.4.22.-
  • HsT17512
  • NEDD8-specific protease 1
  • NEDP1
  • NEDP1NEDD8 specific-protease cysteine 2
  • Protease, cysteine 2
  • protease, cysteine, 2 (NEDD8 specific)
  • PRSC2
  • SENP8
  • Sentrin/SUMO-specific protease SENP8
  • sentrin-specific protease 8
  • SUMO sentrin specific protease family member 8
  • SUMO/sentrin specific peptidase family member 8
  • SUMO/sentrin specific protease family member 8

Background

NEDD8-specific Protease 1 (NEDP1), also known as SUMO/Sentrin Specific Peptidase Family Member 8 (SENP8), DEN1, PRSC2, and FKSG8, is a cytoplasmic cysteine protease that belongs to the ULP/peptidase C48 family of deubiquitinating enzymes (1). It is widely expressed with the highest levels being observed in kidney and pancreas (2). NEDP1/SENP8 is 212 amino acids (aa) in length with a predicted molecular weight of 24.1 kDa (1). It possesses an acetylated initiating methionine plus a protease domain that spans aa 11-174. Human NEDP1/SENP8 shares 92% aa sequence identity with the mouse and rat orthologs. NEDP1/SENP8 is involved in NEDD8-associated ubiquitination (1,2,3-5). NEDD8 is a small Ubiquitin-like protein that covalently binds to target molecules, including Cullin 1 and p53 (6-8). It is a critical regulator of cell proliferation and development (3). NEDP1/SENP8 influences the NEDDylation of proteins by converting pro-NEDD8 into its mature form and deconjugating NEDD8 from its bound substrates (2,3-5,9).

  1. Gan-Erdene, T. et al. (2003) J. Biol. Chem. 278:28892.
  2. Mendoza, H.M. et al. (2003) J. Biol. Chem. 278:25637.
  3. Wu, K. et al. (2003) J. Biol. Chem. 278:28882.
  4. Shen, L. et al. (2005) EMBO J. 24:1341.
  5. Shin, Y.C. et al. (2011) PLoS One 6:e27742.
  6. Pan, Z.Q. et al. (2004) Oncogene 23:1985.
  7. Xirodimas, D.P. et al. (2004) Cell 118:83.
  8. Xirodimas, D.P. et al. (2008) EMBO Rep. 9:280.
  9. Ehrentraut, S.F. et al. (2012) J. Immunol. [Epub ahead of print].

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Bioinformatics

Gene Symbol SENP8
Uniprot