Recombinant Human His6-Livin beta Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Clone
JO1680
Format
Carrier-Free

Order Details

Recombinant Human His6-Livin beta Protein, CF Summary

Details of Functionality
Recombinant Human Livin beta is a Ubiquitin Ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.
Source
E. coli-derived human Livin protein
Contains a C-terminal 6-His tag.
Accession #
Protein/Peptide Type
Recombinant Enzymes
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-Livin beta Protein, CF

  • baculoviral IAP repeat containing 7
  • baculoviral IAP repeat-containing 7
  • BIRC7
  • KIAP
  • KIAPRING finger protein 50
  • Kidney inhibitor of apoptosis protein
  • livin inhibitor-of-apoptosis
  • Livin
  • Melanoma inhibitor of apoptosis protein
  • ML-IAP
  • MLIAPlivin inhibitor of apoptosis
  • ML-IAPmliap
  • RNF50LIVINbaculoviral IAP repeat-containing protein 7

Background

Livin alpha and Livin beta are two splice variants generated from the Baculoviral IAP repeat-containing protein 7 (BIRC7) gene.  Livin in a member of the Inhibitor of Apoptosis Protein (IAP) family that contains a single baculovirus IAP repeat (BIR) and a C-terminal RING domain.  Livin exerts an anti-apoptotic activity by inhibiting caspase's 3, 7, and 9, and by its E3 Ubiquitin ligase activity.  In vitro, Livin directly inhibits caspase 3 and proteolytic activation of pro-caspase 9, and ubiquitinates recombinant DIABLO.  Livin beta is a splice variant that's 18 amino acids shorter in the BIR-RING linking region than the Livin alpha splice variant.
  1. Ashab, Y. et al. (2001) FEBS  495: 56
  2. Kasof, G.M. & Gomes B.C. (2001) J. Biol. Chem.  276: 3238
  3. Lin, J-H. et al. (2000) Biochem. Biophys. Res. Comm.  279: 820
  4. Ma, L. et al. (2006) Cell Death Differ.  13: 2079
  5. Vucic, D. et al. (2000) Curr. Biol.  10: 1359

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Blogs on Livin.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of...  Read full blog post.

Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.

Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.

Livin: On a Prayer
Livin is a member of the inhibitor of apoptosis proteins (IAP) family that regulates programmed cell death. The Livin protein contains a single baculovirus IAP repeat (BIR) essential for function, along with a COOH-terminal RING-type zinc finger domai...  Read full blog post.

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