Recombinant Human His6-HR6B/UBE2B Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

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Recombinant Human His6-HR6B/UBE2B Protein, CF Summary

Details of Functionality
Recombinant Human His6-HR6B/UBE2B is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-HR6B/UBE2B concentration of 0.1-1 μM.
Source
E. coli-derived human HR6B/UBE2B protein
Contains an N-terminal 6-His tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBE2B
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using E2-613.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-HR6B/UBE2B Protein, CF

  • E2 protein
  • E2-17k
  • E2-17kDa
  • hHR6B
  • HR6B
  • RAD6 homolog B
  • RAD6BEC 6.3.2.19
  • UBC2
  • UBE2B
  • Ubiquitin carrier protein B
  • ubiquitin-conjugating enzyme E2 B
  • Ubiquitin-conjugating enzyme E2-17 kDa
  • ubiquitin-conjugating enzyme E2B (RAD6 homolog)
  • Ubiquitin-protein ligase B

Background

Homolog of Rad6 B (HR6B), also known as Ubiquitin-conjugating Enzyme E2B (UBE2B), is a ubiquitously expressed member of the Ubiquitin-conjugating (E2) enzyme family. It has a predicted molecular weight of 17 kDa. The human enzyme shares 100% amino acid sequence identity with the mouse and rat orthologs. HR6B/UBE2B localizes to the nucleus and cytoplasm and is highly expressed in the brain, heart, and testis (1,2). HR6B/UBE2B has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (3). PCNA is mono-ubiquitinated by HR6B/UBE2B via the RAD18 Ubiquitin ligase (E3) (4). HR6B/UBE2B also functions with the UBR2 E3 to ubiquitinate Histone H2A (5). HR6B/UBE2B plays important roles during spermatogenesis and is required for fertility in male mice (6-10). Pathologically, HR6B/UBE2B is overexpressed in mouse and human breast cancer cell lines and tumors and has been reported to provide resistance to chemotherapy drugs (2,11). This protein has an N-terminal His6-tag. (Accession NP_003328)

  1. Koken, M.H. et al. (1996) Dev. Biol. 173:119.
  2. Shekhar, M.P. et al. (2002) Cancer Res. 62:2115.
  3. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  4. Hibbert, R.G. et al. (2011) Proc. Natl. Acad. Sci. USA 108:5590.
  5. An, J.Y. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1912.
  6. Roest, H.P. et al. (1996) Cell 86:799.
  7. Baarends, W.M. et al. (2003) Mol. Cell. Biol. 23:1151.
  8. Mulugeta Achame, E.M. et al. (2010) BMC Genomics 10:367.
  9. Inagaki, A. et al. (2011) J. Cell Sci. 124:2837.
  10. An, J.Y. et al. (2012) PLoS One 7:e37414.
  11. Lyakhovich, A. et al. (2004) Oncogene 23:3097.

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Publications for HR6B/UBE2B (E2-613)(1)

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Bioinformatics

Gene Symbol UBE2B
Uniprot