Recombinant Human His6-HR6A/UBE2A Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

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Recombinant Human His6-HR6A/UBE2A Protein, CF Summary

Details of Functionality
Recombinant Human His6-HR6A/UBE2A is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-HR6A/UBE2A concentration of 0.1-1 μM.
Source
E. coli-derived human HR6A/UBE2A protein
Contains an N-terminal 6-His tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBE2A
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using E2-612.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (XμM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-HR6A/UBE2A Protein, CF

  • EC 6.3.2.19
  • HHR6A
  • HR6A
  • RAD6A
  • RAD6ARAD6 homolog A
  • UBC2
  • UBE2A
  • Ubiquitin carrier protein A
  • ubiquitin-conjugating enzyme E2 A
  • ubiquitin-conjugating enzyme E2A (RAD6 homolog)
  • Ubiquitin-protein ligase A

Background

Homolog of Rad6 A (HR6A), also known as Ubiquitin-conjugating Enzyme E2A (UBE2A), is a ubiquitously expressed member of the Ubiquitin-conjugating (E2) enzyme family that is highly expressed in the brain and heart (1). HR6A/UBE2A has a predicted molecular weight of 17 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. Like other E2 family members, HR6A/UBE2A has an E2 catalytic core domain that contains an active site cysteine residue required for the formation of a thioester bond with Ubiquitin (2). HR6A/UBE2A is involved in many cellular processes including protein degradation, transcriptional regulation, and the DNA damage response. HR6A/UBE2A functions with the UBR2 Ubiquitin ligase (E3) to mediate ubiquitination of target substrates degraded through the N-end rule proteolytic pathway (3). HR6A/UBE2A also interacts with the human BRE1 E3 to ubiquitinate Histone H2B on Lys120, which promotes transcription via methylation of Histone H3 on Lys4 (4). HR6A/UBE2A is activated via phosphorylation on Ser120 by CDK9 following exposure to UVC irradiation and HR6A/UBE2A-RAD18-dependent ubiquitination of PCNA is required for DNA damage tolerance (5-7). HR6A/UBE2A also regulates the mRNA and protein levels of p53 (8). Physiologically, this enzyme is required in female mice for the production of viable offspring (9). Mutations in human HR6A/UBE2A cause UBE2A deficiency syndrome in males, which is characterized by intellectual disability, seizures, absent speech, and urogenital and skin anomalies (10-12). This protein has an N-terminal His6-tag. (Accession NP_003327)

  1. Koken, M.H. et al. (1996) Dev. Biol. 173:119.
  2. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  3. Lee, P.C. et al. (2011) Mol. Cell 43:392.
  4. Kim, J. et al. (2009) Cell 137:459.
  5. Unk, I. et al. (2006) Proc. Natl. Acad. Sci. USA 103:18107.
  6. Yoon, J.H. et al. (2012) Proc. Natl. Acad. Sci. USA 109:7799.
  7. Shchebet, A. et al. (2012) Cell Cycle 11:2122.
  8. Chen, S. et al. (2012) Mol. Cell. Biol. 32:576.
  9. Roest, H.P. et al. (2004) Mol. Cell. Biol. 24:5485.
  10. Nascimento, R.M. et al. (2006) Am. J. Hum. Genet. 79:549.
  11. Honda, S. et al. (2010) J. Hum. Genet. 55:244.
  12. de Leeuw, N. et al. (2010) Am. J. Med. Genet. 152A:3084.
 

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Publications for HR6A/UBE2A (E2-612)(1)

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol UBE2A
Entrez
Uniprot