Recombinant Human His6-HR23A/Rad23A Tandem UBA (TUBE1), CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human His6-HR23A/Rad23A Tandem UBA (TUBE1), CF Summary

Details of Functionality
His6-TUBE1 can be used for the purification and identification of K63- or K48-linked Ubiquitin chains. Reaction conditions will need to be optimized for each specific application. We recommend using 50-100 μg of His6-TUBE1 to detect 10-20 μg of purified Ubiquitin chains.
Source
E. coli-derived
Accession #
Protein/Peptide Type
Affinity Matrices
Gene
RAD23A
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-HR23A/Rad23A Tandem UBA (TUBE1), CF

  • HR23A
  • Rad23A

Background

Human HR23A/Rad23A has two Ubiquitin-associated (UBA) motifs that can each bind Ubiquitin via a hydrophobic surface formed by residues located within the alpha 1 and alpha 3 helices of each UBA domain (1,2). Tandem-repeated Ubiquitin binding entities (TUBEs), which consist of multiple tandem Ubiquitin-binding UBA motifs, have been developed for the isolation and identification of ubiquitinated proteins (3). TUBEs show increased affinity for poly-Ubiquitin moieties compared to single UBA motifs (4). Additionally, TUBEs protect polyubiquitinated proteins from deubiquitylating enzymes, allowing for the detection of polyubiquitinated proteins at relatively low levels of abundance (5).

 

This protein is His6-tagged which allows for metal chelate affinity purification and also allows for convenient immuno-detection of conjugates using His6-specific antibodies.

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Bioinformatics

Gene Symbol RAD23A
Uniprot