Recombinant Human His6-GATE-16 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human His6-GATE-16 Protein, CF Summary

Details of Functionality
Recombinant Human His6-GATE-16/Apg8p2 can be conjugated to substrate proteins via the subsequent actions of an Apg-activating (E1) enzyme, an Apg-conjugating (E2) enzyme, and an Apg ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-GATE-16/Apg8p2 concentration of 10-50 μM.
Source
E. coli-derived human GATE-16 protein
Contains a 6-His tag
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
GABARAPL2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer

X mg/ml (XμM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 1 mM DTT. Concentration varies with lot number.

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-GATE-16 Protein, CF

  • Apg8p2
  • ATG8
  • ATG8C
  • FLC3A
  • GABARAPL2
  • gamma-aminobutyric acid receptor-associated protein-like 2
  • Ganglioside expression factor 2
  • GATE16
  • GATE-16
  • GEF2
  • GEF-2
  • GEF2GEF-2
  • General Protein Transport Factor P16
  • Golgi-Associated ATPase Enhancer Of 16 KDa
  • MAP1 light chain 3 related protein
  • MAP1 light chain 3-related protein

Background

Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as Apg8p2 and GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the Autophagy-related 8 (Atg8) family of proteins (1). GATE-16/Apg8p2 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GATE-16/Apg8p2 is best known for its role in autophagy (2,3). GATE-16/Apg8p2 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).

  1. Shpilka, T. et al. (2011) Genome Biol. 12:226.
  2. Wang, H. et al. (1999) Nature 397:69.
  3. Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
  4. Weidberg, H. et al. (2010) EMBO J. 29:1792.
  5. Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125

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FAQs for GATE-16/GABARAPL2 (UL-420). (Showing 1 - 1 of 1 FAQ).

  1. I am currently interested in detecting GABARAPL2 by immunofluorescence. Do you have an antibody that I could try out for immunofluorescence
    • Here is the link using our search bar and filters: http://www.novusbio.com/productsearch/GABARAPL2#fq=common_name%3A%22GABARAPL2%22&fq=applications%3A%22Immunofluorescence%22

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Bioinformatics

Gene Symbol GABARAPL2
Uniprot