Recombinant Human HA-GATE-16 Vinyl Sulfone Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human HA-GATE-16 Vinyl Sulfone Protein, CF Summary

Details of Functionality
Add Recombinant Human HA-GATE-16/Apg8p2 Vinyl Sulfone to in vitro assays to inhibit Apg8-specific isopeptidases. The HA-tag allows for detection and purification of Apg8-specific isopeptidases activity. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human HA-GATE-16/Apg8p2 Vinyl Sulfone concentration of 0.1-1 μM.
Source
E. coli-derived human GATE-16 protein
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
GABARAPL2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Packaging, Storage & Formulations

Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HA-GATE-16 Vinyl Sulfone Protein, CF

  • Apg8p2
  • ATG8
  • ATG8C
  • FLC3A
  • GABARAPL2
  • gamma-aminobutyric acid receptor-associated protein-like 2
  • Ganglioside expression factor 2
  • GATE16
  • GATE-16
  • GEF2
  • GEF-2
  • GEF2GEF-2
  • General Protein Transport Factor P16
  • Golgi-Associated ATPase Enhancer Of 16 KDa
  • MAP1 light chain 3 related protein
  • MAP1 light chain 3-related protein

Background

Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as Apg8p2 and GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the Autophagy-related 8 (Atg8) family of proteins. GATE-16/Apg8p2 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GATE-16/Apg8p2 is best known for its role in autophagy. GATE-16/Apg8p2 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation. It may also be involved in cargo recruitment to autophagosomes.

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FAQs for GATE-16/GABARAPL2 (UL-446). (Showing 1 - 1 of 1 FAQ).

  1. I am currently interested in detecting GABARAPL2 by immunofluorescence. Do you have an antibody that I could try out for immunofluorescence
    • Here is the link using our search bar and filters: http://www.novusbio.com/productsearch/GABARAPL2#fq=common_name%3A%22GABARAPL2%22&fq=applications%3A%22Immunofluorescence%22

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Bioinformatics

Gene Symbol GABARAPL2
Entrez
Uniprot