Recombinant Human HA-GATE-16 AMC Protein, CF

Images

 
There are currently no images for GATE-16/GABARAPL2 (UL-445).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human HA-GATE-16 AMC Protein, CF Summary

Details of Functionality
Recombinant Human HA-GATE-16 AMC is a fluorogenic substrate for some ATG-specific isopeptidases.  Release of AMC fluorescence can be monitored with an excitation wavelength of 345 nM and an emission wavelength of 445 nM. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human HA-GATE-16 AMC concentration of 0.1-1 μM.
Source
E. coli-derived human GATE-16 protein
Contains an N-terminal HA (YPYDVPDYA) tag
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
GABARAPL2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Applications/Dilutions

Theoretical MW

14 kDa

.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
UL-445 in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer

X mg/ml (X µM) in 50 mM MOPS pH 6.5, 100 mM NaCl

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HA-GATE-16 AMC Protein, CF

  • Apg8p2
  • ATG8
  • ATG8C
  • FLC3A
  • GABARAPL2
  • gamma-aminobutyric acid receptor-associated protein-like 2
  • Ganglioside expression factor 2
  • GATE16
  • GATE-16
  • GEF2
  • GEF-2
  • GEF2GEF-2
  • General Protein Transport Factor P16
  • Golgi-Associated ATPase Enhancer Of 16 KDa
  • MAP1 light chain 3 related protein
  • MAP1 light chain 3-related protein

Background

Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the Autophagy-related 8 (ATG8) family of proteins (1). GATE-16 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast ATG8. ATG8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GATE-16 is best known for its role in autophagy (2,3). GATE-16 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).

 

This is a fluorogenic substrate for ATG8-specific C-terminal hydrolases such as ATG4B (Catalog # E-400) based on the C-terminal derivatization of ATG8 with 7-amido-4-methylcoumarin (AMC). ATG8-AMC is useful for studying such enzyme activities when detection sensitivity or continuous monitoring of activity is essential. NOTE: this protein has an N-terminal HA tag.

  1. Shpilka, T. et al. (2011) Genome Biol. 12:226.
  2. Wang, H. et al. (1999) Nature 397:69.
  3. Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
  4. Weidberg, H. et al. (2010) EMBO J. 29:1792.
  5. Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125.

Customers Who Viewed This Item Also Viewed...

NB100-2331
Species: Hu, Mu, Rt, Ca, Fi, Pl, Ze
Applications: WB, Simple Western, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, SB, IHC-WhMt
NBP1-71771
Species: Hu, Mu, Dr
Applications: WB, ICC/IF, IHC, IHC-P
MAB6608
Species: Hu, Mu
Applications: WB, Simple Western, IHC, CyTOF-ready, ICC, ICFlow
NBP1-55202
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-15501
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
NB110-53818
Species: Hu, Mu, Rt, Po, Bv, Fi, Gp, Pm, Xp, Ze
Applications: WB, Simple Western, EM, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, PLA, RIA, KD, KO
NBP2-01083
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
H00008878-M01
Species: Hu, Mu, Rt, Bv, Ha, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, S-ELISA
NBP2-24709
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-33950
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24738
Species: Hu, Mu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89228
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB500-249
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
H00002965-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, RNAi, S-ELISA
NBP1-31381
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-37399
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NBP2-02627
Species: Hu, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-19151
Species: Hu, Bv, Ma
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-2220
Species: Hu, Mu, Rt, Po, Av, Ba, Bv, Ca, Ch, SyHa, Gp, Ha, In, Pm, Pm, Rb, Ze
Applications: WB, Simple Western, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, ChIP, KD, KO
AF9024
Species: Mu, Rt
Applications: WB, IHC

Publications for GATE-16/GABARAPL2 (UL-445)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for GATE-16/GABARAPL2 (UL-445) (0)

There are no reviews for GATE-16/GABARAPL2 (UL-445). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for GATE-16/GABARAPL2 (UL-445). (Showing 1 - 1 of 1 FAQ).

  1. I am currently interested in detecting GABARAPL2 by immunofluorescence. Do you have an antibody that I could try out for immunofluorescence
    • Here is the link using our search bar and filters: http://www.novusbio.com/productsearch/GABARAPL2#fq=common_name%3A%22GABARAPL2%22&fq=applications%3A%22Immunofluorescence%22

Other Available Formats

Additional GATE-16/GABARAPL2 Products

Bioinformatics Tool for GATE-16/GABARAPL2 (UL-445)

Discover related pathways, diseases and genes to GATE-16/GABARAPL2 (UL-445). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GATE-16/GABARAPL2 (UL-445)

Discover more about diseases related to GATE-16/GABARAPL2 (UL-445).
 

Pathways for GATE-16/GABARAPL2 (UL-445)

View related products by pathway.

PTMs for GATE-16/GABARAPL2 (UL-445)

Learn more about PTMs related to GATE-16/GABARAPL2 (UL-445).

Blogs on GATE-16/GABARAPL2

There are no specific blogs for GATE-16/GABARAPL2, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

LC3B Antibody
NB100-2220
LC3A Antibody
NB100-2331

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human HA-GATE-16 AMC Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol GABARAPL2
Uniprot