Recombinant Human HA-GATE-16 AMC Protein, CF Summary
Details of Functionality
Recombinant Human HA-GATE-16 AMC is a fluorogenic substrate for some ATG-specific isopeptidases. Release of AMC fluorescence can be monitored with an excitation wavelength of 345 nM and an emission wavelength of 445 nM. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human HA-GATE-16 AMC concentration of 0.1-1 μM.
E. coli-derived human GATE-16 protein Contains an N-terminal HA (YPYDVPDYA) tag
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using UL-445 in the following applications:
Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the Autophagy-related 8 (ATG8) family of proteins (1). GATE-16 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast ATG8. ATG8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GATE-16 is best known for its role in autophagy (2,3). GATE-16 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).
This is a fluorogenic substrate for ATG8-specific C-terminal hydrolases such as ATG4B (Catalog # E-400) based on the C-terminal derivatization of ATG8 with 7-amido-4-methylcoumarin (AMC). ATG8-AMC is useful for studying such enzyme activities when detection sensitivity or continuous monitoring of activity is essential. NOTE: this protein has an N-terminal HA tag.
Shpilka, T. et al. (2011) Genome Biol. 12:226.
Wang, H. et al. (1999) Nature 397:69.
Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
Weidberg, H. et al. (2010) EMBO J. 29:1792.
Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Recombinant Human HA-GATE-16 AMC Protein, CF and receive a gift card or discount.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.