Recombinant Human GST-UBE2M/Ubc12 Protein, CF

Images

 
There are currently no images for UBE2M/Ubc12 (E2-655).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human GST-UBE2M/Ubc12 Protein, CF Summary

Details of Functionality
Recombinant Human GST-UBE2M/Ubc12 is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-UBE2M/Ubc12 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2M/Ubc12 protein
Contains an N-terminal GST (glutathione S-transferase) tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBE2M
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GST-UBE2M/Ubc12 Protein, CF

  • EC 6.3.2
  • EC 6.3.2.-
  • hUbc12
  • NEDD8 carrier protein
  • NEDD8 protein ligase
  • UBC12
  • UBC12NEDD8-conjugating enzyme Ubc12
  • UbcH12
  • UBC-RS2
  • UBE2M
  • ubiquitin carrier protein M
  • Ubiquitin-conjugating enzyme E2 M
  • ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12)
  • ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
  • ubiquitin-protein ligase M
  • yeast UBC12 homolog

Background

Ubiquitin-conjugating Enzyme E2M (UBE2M), also known as Ubiquitin-conjugating Enzyme 12 (Ubc12), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. UBE2M/Ubc12 is highly conserved, and the human ortholog shares 100% amino acid identity with mouse and rat UBE2M. UBE2M/Ubc12 mediates conjugation of the Ubiquitin-like protein Neural Precursor Cell Expressed, Developmentally Down-regulated 8 (NEDD8) to substrate proteins (1). Neddylation requires the coordinated activities of UBE2M/Ubc12 and the APPBPI/UBA3 heterodimer, a NEDD8-activating (E1) enzyme (2,3). The NEDD8 pathway is essential for the viability of many organisms and plays important roles in morphogenesis and cell cycle progression (4-5). UBE2M/Ubc12-dependent neddylation modulates the levels of p27/Kip1, beta-Catenin, Cdc53/Cul1, and other members of the Cullin family (6-9).

  1. Gong, L. et al. (1999) J. Biol. Chem. 274:12036. 
  2. Huang, D.T. et al. (2005) Mol. Cell 17:341. 
  3. Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.
  4. Osaka, F. et al. (2000) EMBO J. 19:3475.
  5. Tateishi, K. et al. (2001) J. Cell Biol. 155:571. 
  6. Huang, A.M. et al. (2011) Biochem. Pharmacol. 82:35.
  7. Miller, B.W. et al. (2009) Mol. Syst. Biol. 5:315.
  8. Huang, D.T. et al. (2009) Mol. Cell 33:483.
  9. Tanaka, T. et al. (2012) Mol. Oncol. 6:267.

Customers Who Viewed This Item Also Viewed...

NBP2-37523
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
NB120-1868
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
AF2574
Species: Hu
Applications: CyTOF-ready, ICFlow
H00143384-P01
Species: Hu
NBP2-49443
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-03523
Species: Hu, Ca
Applications: WB, Flow
NBP2-47645
Species: Hu, Mu, Rt
Applications: Flow, IHC-P, PAGE, IF
NBP2-13961
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88742
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB7675
Species: Hu
Applications: WB, Simple Western, ICC
NB100-56583
Species: Hu
Applications: WB, Flow, IHC-P
H00054165-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NB100-2267
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NBP1-58906
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
E2-655
Species: Hu
Applications: Enzyme Activity

Publications for UBE2M/Ubc12 (E2-655) (0)

There are no publications for UBE2M/Ubc12 (E2-655).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for UBE2M/Ubc12 (E2-655) (0)

There are no reviews for UBE2M/Ubc12 (E2-655). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for UBE2M/Ubc12 (E2-655) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for UBE2M/Ubc12 (E2-655)

Discover related pathways, diseases and genes to UBE2M/Ubc12 (E2-655). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for UBE2M/Ubc12 (E2-655)

Discover more about diseases related to UBE2M/Ubc12 (E2-655).
 

Pathways for UBE2M/Ubc12 (E2-655)

View related products by pathway.

Blogs on UBE2M/Ubc12

There are no specific blogs for UBE2M/Ubc12, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human GST-UBE2M/Ubc12 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol UBE2M
Entrez
Uniprot