Recombinant Human Flt-3 Ligand Protein, CF

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1 µg/lane of Recombinant Human Flt-3 Ligand was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at24‑28 kDa. Multiple bands are due to variable ...read more
Recombinant Human Flt-3 Ligand (Catalog # 308-FKN/CF) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Flt-3 Ligand Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.
The specific activity of Recombinant Human Flt-3 Ligand is approximately 1.2 x 103 U/μg, which is calibrated against recombinant human
Flt-3 Ligand WHO Standard (NIBSC code: 96/532).
Source
Mouse myeloma cell line, NS0-derived human Flt-3 Ligand protein
Thr27-Pro185
Accession #
N-terminal Sequence
Thr27
Protein/Peptide Type
Recombinant Proteins
Gene
FLT3LG
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
Multiple bands between 24 kDa and 28 kDa, reducing conditions
Publications
Read Publications using
308-FKN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute 5 µg vials at 50 µg/mL in sterile PBS. Reconstitute 25 µg or larger vials at 100 µg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Flt-3 Ligand Protein, CF

  • FL
  • Flt3 ligand
  • Flt-3 Ligand
  • Flt3L
  • FLT3LG
  • fms-related tyrosine kinase 3 ligand
  • SL cytokine

Background

Flt-3/Flk-2 Ligand (FL) is a hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt-3/Flk-2. Flt-3/Flk-2 was first discovered as a member of the class III subfamily of receptor tyrosine kinases (RTK) whose expression among hematopoietic cells was found to be restricted to highly enriched stem/progenitor cell populations. Additional class III RTKs include the receptors from SCF, M-CSF and PDGF. Not surprisingly, Flt-3/Flk-2 Ligand is also structurally related to M-CSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins. The predominant human FL isoform is a transmembrane protein that can undergo proteolytic cleavage to generate a soluble form of the protein. An alternatively-spliced FL mRNA, encoding a soluble form of the human FL, has also been identified. FL is widely expressed in various human and mouse tissues. At the amino acid sequence level, human and mouse FL are approximately 72% identical and the two proteins exhibit cross-species activity. FL has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors.

  1. Lyman, S.D. (1995) International Journal of Hematology 62:63.

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308-FKN/CF
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Applications: Bioactivity

Publications for Flt-3 Ligand (308-FKN/CF)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(10)
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Filter By Species
Human
(9)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using 308-FKN/CF Applications Species
Smith A, Bowers S, Stratman A, Davis G Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions. PLoS ONE, 2013;8(12):e85147. 2013 [PMID: 24391990] (Bioassay, Human) Bioassay Human
Ichii, Michiko, Frank, Mark Bar, Iozzo, Renato V, Kincade, Paul W The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells. Blood, 2012;119(7):1683-92. 2012 [PMID: 22117039] (Bioassay, Human) Bioassay Human
Uchida N, Hsieh MM, Hayakawa J, Madison C, Washington KN, Tisdale JF Optimal conditions for lentiviral transduction of engrafting human CD34+ cells. Gene Ther., 2011;18(11):1078-86. 2011 [PMID: 21544097] (Bioassay, Human) Bioassay Human
Tolar J, Park I, Xia L, Lees C, Peacock B, Webber B, McElmurry R, Eide C, Orchard P, Kyba M, Osborn M, Lund T, Wagner J, Daley G, Blazar B Hematopoietic differentiation of induced pluripotent stem cells from patients with mucopolysaccharidosis type I (Hurler syndrome). Blood, 2011;117(3):839-47. 2011 [PMID: 21037085] (Bioassay, Human) Bioassay Human
Trowbridge JJ, Guezguez B, Moon RT Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity. Stem Cells, 2010;28(8):1379-89. 2010 [PMID: 20521329] (Bioassay, Mouse) Bioassay Mouse
Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells. Exp. Hematol., 2008;36(9):1121-31. 2008 [PMID: 18562079] (Bioassay, Human) Bioassay Human
McCullar V, Oostendorp R, Panoskaltsis-Mortari A, Yun G, Lutz CT, Wagner JE, Miller JS Mouse fetal and embryonic liver cells differentiate human umbilical cord blood progenitors into CD56-negative natural killer cell precursors in the absence of interleukin-15. Exp. Hematol., 2008;36(5):598-608. 2008 [PMID: 18295962] (Bioassay, Human) Bioassay Human
Gemelli C, Montanari M, Tenedini E, Zanocco Marani T, Vignudelli T, Siena M, Zini R, Salati S, Tagliafico E, Manfredini R, Grande A, Ferrari S Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells. Cell Death Differ., 2006;13(10):1686-96. 2006 [PMID: 16456583] (Bioassay, Human) Bioassay Human
Kersten C, Dosen G, Myklebust JH, Sivertsen EA, Hystad ME, Smeland EB, Rian E BMP-6 inhibits human bone marrow B lymphopoiesis--upregulation of Id1 and Id3. Exp. Hematol., 2006;34(1):72-81. 2006 [PMID: 16413393] (Bioassay, Human) Bioassay Human
Manz MG, Miyamoto T, Akashi K, Weissman IL Prospective isolation of human clonogenic common myeloid progenitors. Proc. Natl. Acad. Sci. U.S.A., 2002;99(18):11872-7. 2002 [PMID: 12193648] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol FLT3LG
Uniprot