Recombinant Human FLAG-USP36 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human FLAG-USP36 Protein, CF Summary

• Details of Functionality: Recombinant Human FLAG-USP36 is a Ubiquitin deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial concentration of 10-100 nM USP36 when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine (U-555) as a substrate.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP36 protein
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: X mg/ml (X μM) in 50 mM HEPES pH 7.5, 300 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FLAG-USP36 Protein, CF

• Deubiquitinating enzyme 36
• DUB1
• EC 3.1.2.15
• EC 3.4.19.12
• FLJ12851
• KIAA1453
• KIAA1453deubiquitinating enzyme 1
• ubiquitin carboxyl-terminal hydrolase 36
• ubiquitin specific peptidase 36
• ubiquitin specific protease 36
• ubiquitin thioesterase 36
• Ubiquitin thiolesterase 36
• Ubiquitin-specific-processing protease 36
• USP36

Background

Ubiquitin carboxyl-terminal hydrolase 36 (USP36) is a C19 type peptidase with a predicted molecular weight of 123 kDa.  The human protein shares 72% amino acid sequence identity with its mouse ortholog.  By stabilizing c-Myc and SOD2 proteins, USP36 protects human renal HK-2 cells from ischemic apoptosis in an in vitro model of acute kidney injury.  In vitro, USP36 hydrolyzes all polyubiquitin chain linkage types other than K27.  This recombinant protein contains a c-terminal 2X FLAG tag. 

1. Liu Q et al.  (2019) Biochem. Biophys. Res. Comm.  513: 502
2. Sun X-X, Sears R.C. & Dai M-S  (2015) Cell Cycle  14: 3786