Recombinant Human FLAG-USP36 Protein, CF Summary
Details of Functionality |
Recombinant Human FLAG-USP36 is a Ubiquitin deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial concentration of 10-100 nM USP36 when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine (U-555) as a substrate.
|
Source |
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP36 protein
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Protein/Peptide Type |
Recombinant Enzymes |
Purity |
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
Applications/Dilutions
Theoretical MW |
125 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 60 months from date of receipt, -70 °C as supplied.
- 3 months, -70 °C under sterile conditions after opening.
|
Buffer |
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 300 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
|
Purity |
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human FLAG-USP36 Protein, CF
Background
Ubiquitin carboxyl-terminal hydrolase 36 (USP36) is a C19 type peptidase with a predicted molecular weight of 123 kDa. The human protein shares 72% amino acid sequence identity with its mouse ortholog. By stabilizing c-Myc and SOD2 proteins, USP36 protects human renal HK-2 cells from ischemic apoptosis in an in vitro model of acute kidney injury. In vitro, USP36 hydrolyzes all polyubiquitin chain linkage types other than K27. This recombinant protein contains a c-terminal 2X FLAG tag.
- Liu Q et al. (2019) Biochem. Biophys. Res. Comm. 513: 502
- Sun X-X, Sears R.C. & Dai M-S (2015) Cell Cycle 14: 3786
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