Recombinant Human FLAG (DYKDDDDK)-Ubiquitin Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human FLAG (DYKDDDDK)-Ubiquitin Protein, CF Summary

Details of Functionality
Recombinant Human FLAG-Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human FLAG-Ubiquitin concentration of 0.5-1 mM.
Source
E. coli-derived human Ubiquitin protein Contains an N-terminal FLAG (DYKDDDDK) tag
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
UBB
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
9.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
U-120 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Buffer
Lyophilized from a solution in deionized water.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Reconstitution Instructions
Reconstitute at 10 mg/mL in an aqueous solution.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FLAG (DYKDDDDK)-Ubiquitin Protein, CF

  • RPS27A
  • UBA52
  • UBB ubiquitin B
  • UBB
  • UBC
  • Ubiquitin

Background

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7). This fully functional N-terminal FLAG-tagged Ubiquitin protein allows for the convenient detection or affinity purification of ubiquitinated proteins in vitro. The epitope tag is a hydrophilic octapeptide (DYKDDDDK) derived from the sequence of the bacteriophage 7 gene-10 product. This tag is specifically recognized by Anti-FLAG antibodies M1, M2 or M5.
  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.

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U-120
Species: Hu
Applications: Enzyme Activity

Publications for Ubiquitin (U-120)(11)

We have publications tested in 4 confirmed species: Human, N/A, Xenopus, Yeast.

We have publications tested in 3 applications: Bioassay, In Vivo, Ubiquitination.


Filter By Application
Bioassay
(8)
In Vivo
(1)
Ubiquitination
(1)
All Applications
Filter By Species
Human
(2)
N/A
(2)
Xenopus
(1)
Yeast
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using U-120 Applications Species
LY Jiang, W Jiang, N Tian, YN Xiong, J Liu, J Wei, KY Wu, J Luo, XJ Shi, BL Song Ring finger protein 145 (RNF145) is a ubiquitin ligase for sterol-induced degradation of HMG-CoA reductase J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29374057] (Bioassay) Bioassay
N Lian, X Liu, X Wang, Y Zhou, H Li, J Li, T Mao COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongation Proc. Natl. Acad. Sci. U.S.A., 2017;114(46):12321-12326. 2017 [PMID: 29087315] (Bioassay, Human) Bioassay Human
EE Blythe, KC Olson, V Chau, RJ Deshaies Ubiquitin- and ATP-dependent unfoldase activity of P97/VCP�NPLOC4�UFD1L is enhanced by a mutation that causes multisystem proteinopathy Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28512218] (Bioassay) Bioassay
R Tawo, W Pokrzywa, É Kevei, ME Akyuz, V Balaji, S Adrian, J Höhfeld, T Hoppe The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor Turnover Cell, 2017;169(3):470-482.e13. 2017 [PMID: 28431247] (Bioassay) Bioassay
TJ Kucharski, PE Minshall, M Moustafa-K, AS Turnell, JG Teodoro Reciprocal Regulation between 53BP1 and the Anaphase-Promoting Complex/Cyclosome Is Required for Genomic Stability during Mitotic Stress Cell Rep, 2017;18(8):1982-1995. 2017 [PMID: 28228263]
C Liu, W Liu, Y Ye, W Li Ufd2p synthesizes branched ubiquitin chains to promote the degradation of substrates modified with atypical chains Nat Commun, 2017;8(0):14274. 2017 [PMID: 28165462] (Bioassay, Yeast) Bioassay Yeast
Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1 Elife, 2016;5(0):. 2016 [PMID: 27595565] (Ubiquitination, Human) Ubiquitination Human
Rodrigo-Brenni M, Gutierrez E, Hegde R Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6. Mol Cell, 2014;55(2):227-37. 2014 [PMID: 24981174] (Bioassay) Bioassay
Kumar, Ravindra, Whitehurst, Christop, Pagano, Joseph S The Rad6/18 ubiquitin complex interacts with the Epstein-Barr virus deubiquitinating enzyme, BPLF1, and contributes to virus infectivity. J Virol, 2014;88(11):6411-22. 2014 [PMID: 24672041] (Bioassay, N/A) Bioassay N/A
Liu, Weixiao, Shang, Yonglian, Zeng, Yan, Liu, Chao, Li, Yanchang, Zhai, Linhui, Wang, Pan, Lou, Jizhong, Xu, Ping, Ye, Yihong, Li, Wei Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains. EMBO J, 2014;33(1):46-61. 2014 [PMID: 24366945] (Bioassay, N/A) Bioassay N/A
Show All 11 Publications.

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Blogs on Ubiquitin.

There's an autophagy for that!
By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

PINK1: All work and no fun
The protein PINK1 is a mitochondrial-located serine/threonine kinase (PTK) that maintains organelle function and integrity. It not only protects organelles from cellular stress, but it also uses the selective auto-phagocytosis process for cleaning and...  Read full blog post.

Ubiquitin-Mediated Degradation of Cellular Proteins: The Kiss of Death
Ubiquitin is an abundant and essential cellular 9-kd protein that is conserved across evolution from yeast to humans. Ubiquitin is used by cells as a covalent modifier of other proteins both to activate their function and to target them for degradatio...  Read full blog post.

Using Ubiquitin Antibodies in Various Disease Research
Ubiquitin is a small, highly conserved protein which plays an important role in protein breakdown, covalently bonding to proteins to mark them for proteolytic degradation in a process called ubiquitination. Ubiquitin also binds to inclusion bodies (ac...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

The Latest Research on IBR-type E3 Ubiquitin Ligases
E3 ubiquitin ligases are standards in most antibody catalogs. These proteins are essential to the process of ubiquitination, which is expressed in protein pathways throughout the body and is often linked to disease states. It is widely used as a bioma...  Read full blog post.

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Bioinformatics

Gene Symbol UBB
Entrez
Uniprot