Recombinant Human FGF basic/FGF2 (146 aa) Protein, CF

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1 µg/lane of Recombinant Human FGF basic/FGF2 (146 aa) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human FGF basic/FGF2 (146 aa) (Catalog # 233-FB/CF) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.1-0.6 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human FGF basic/FGF2 (146 aa) Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.
The specific activity of Recombinant Human FGF basic/FGF2  is approximately 800 IU/μg, which is calibrated against recombinant human FGF basic/FGF2  WHO International Standard (NIBSC code: 90/712).
Source
E. coli-derived human FGF basic/FGF2 protein
Pro143-Ser288, with an N-terminal Ala
Accession #
N-terminal Sequence
Ala-Pro143
Protein/Peptide Type
Recombinant Proteins
Gene
FGF2
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
233-FB/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF basic/FGF2 (146 aa) Protein, CF

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGF2AS
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • GFG1
  • HBGF-2
  • HBGH-2
  • heparin-binding growth factor 2
  • NUDT6
  • Prostatropin

Background

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. A variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration. FGF basic also modulates embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

  1. Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
  2. Chen, C.H. et al. (2004) Curr. Vasc. Pharmacol. 2:33.
  3. Mohammadi, M. et al. (2005) Curr. Opin. Struct. Biol. 15:506.
  4. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.

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Applications: Bioactivity

Publications for FGF basic/FGF2 (233-FB/CF)(308)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Hamster, Humane, N/A, Porcine, Primate - Callitrix jacchus (Common Marmoset), Xenograft, Zebrafish.

We have publications tested in 6 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, ELISA (Standard), In Vivo.


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Binding Assay
(1)
Bioassay
(289)
Cell Culture
(3)
Differentiation
(1)
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(1)
In Vivo
(9)
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Human
(234)
Mouse
(37)
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(10)
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(4)
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(3)
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(6)
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(1)
Humane
(1)
N/A
(1)
Porcine
(4)
Primate - Callitrix jacchus (Common Marmoset)
(1)
Xenograft
(1)
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Showing Publications 1 - 10 of 308. Show All 308 Publications.
Publications using 233-FB/CF Applications Species
A Hall, K Choi, W Liu, J Rose, C Zhao, Y Yu, Y Na, Y Cai, RA Coover, Y Lin, E Dombi, M Kim, D Levanon, Y Groner, E Boscolo, D Pan, PP Liu, QR Lu, N Ratner, G Huang, J Wu RUNX represses Pmp22 to drive neurofibromagenesis Sci Adv, 2019;5(4):eaau8389. 2019 [PMID: 31032403] (Bioassay, Mouse) Bioassay Mouse
EE Mulkearns-, LA Torre-Heal, DJ Silver, JT Eurich, D Bayik, E Serbinowsk, M Hitomi, J Zhou, B Przychodze, R Zhang, SA Sprowls, JS Hale, TJ Alban, A Berezovsky, BA Bell, PR Lockman, BK Jha, JD Lathia Development of a Cx46 Targeting Strategy for Cancer Stem Cells Cell Rep, 2019;27(4):1062-1072.e5. 2019 [PMID: 31018124] (Bioassay, Xenograft) Bioassay Xenograft
L Sun, F Wang, H Chen, D Liu, T Qu, X Li, D Xu, F Liu, Z Yin, Y Chen Co-Transplantation of Human Umbilical Cord Mesenchymal Stem Cells and Human Neural Stem Cells Improves the Outcome in Rats with Spinal Cord Injury Cell Transplant, 2019;0(0):9636897198445. 2019 [PMID: 31012325] (Bioassay, Human) Bioassay Human
M Urbischek, H Rannikmae, T Foets, K Ravn, M Hyvönen, M de la Roch Organoid culture media formulated with growth factors of defined cellular activity Sci Rep, 2019;9(1):6193. 2019 [PMID: 30996238] (Bioassay, Human) Bioassay Human
G MacLeod, DA Bozek, N Rajakulend, V Monteiro, M Ahmadi, Z Steinhart, MM Kushida, H Yu, FJ Coutinho, FMG Cavalli, I Restall, X Hao, T Hart, HA Luchman, S Weiss, PB Dirks, S Angers Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells Cell Rep, 2019;27(3):971-986.e9. 2019 [PMID: 30995489] (Bioassay, Human) Bioassay Human
T Ryynänen, A Pelkonen, K Grigoras, OME Ylivaara, T Hyvärinen, J Ahopelto, M Prunnila, S Narkilahti, J Lekkala Microelectrode Array With Transparent ALD TiN Electrodes Front Neurosci, 2019;13(0):226. 2019 [PMID: 30967754] (Bioassay, Human) Bioassay Human
S Varum, A Baggiolini, L Zurkirchen, ZK Atak, C Cantù, E Marzorati, R Bossart, J Wouters, J Häusel, E Tuncer, D Zingg, D Veen, N John, M Balz, MP Levesque, K Basler, S Aerts, N Zamboni, R Dummer, L Sommer Yin Yang 1 Orchestrates a Metabolic Program Required for Both Neural Crest Development and Melanoma Formation Cell Stem Cell, 2019;24(4):637-653.e9. 2019 [PMID: 30951662] (Bioassay, Mouse) Bioassay Mouse
M Matsushita, Y Mori, K Uchiumi, T Ogata, M Nakamura, H Yoda, H Soda, N Takiguchi, Y Nabeya, O Shimozato, T Ozaki PTPRK suppresses progression and chemo-resistance of colon cancer cells via direct inhibition of pro-oncogenic CD133 FEBS Open Bio, 2019;0(0):. 2019 [PMID: 30947381] (Bioassay, Human) Bioassay Human
M Rutgers, F Bach, L Vonk, M van Rijen, V Akrum, A van Boxtel, W Dhert, L Creemers PTH decreases in vitro human cartilage regeneration without affecting hypertrophic differentiation PLoS ONE, 2019;14(4):e0213483. 2019 [PMID: 30947269] (Bioassay, Human) Bioassay Human
HL Zhang, P Wang, MZ Lu, SD Zhang, L Zheng c-Myc maintains the self-renewal and chemoresistance properties of colon cancer stem cells Oncol Lett, 2019;17(5):4487-4493. 2019 [PMID: 30944638] (Bioassay, Human) Bioassay Human
Show All 308 Publications.

Reviews for FGF basic/FGF2 (233-FB/CF) (0)

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FAQs for FGF basic/FGF2 (233-FB/CF). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

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Bioinformatics

Gene Symbol FGF2
Uniprot