Recombinant Human FGF basic (146 aa) Protein

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1 µg/lane of Recombinant Human FGF basic (146 aa) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human FGF basic (146aa) (Catalog # 233-FB) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.1-0.6 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human FGF basic (146 aa) Protein Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.
The specific activity of Recombinant Human FGF basic is approximately 800 IU/μg, which is calibrated against recombinant human FGF basic WHO International Standard (NIBSC code: 90/712).
Source
E. coli-derived human FGF basic protein
Pro143-Ser288, with an N-terminal Ala
Accession #
N-terminal Sequence
Ala-Pro143
Protein/Peptide Type
Recombinant Proteins
Gene
FGF2
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
233-FB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF basic (146 aa) Protein

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGF2AS
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • GFG1
  • HBGF-2
  • HBGH-2
  • heparin-binding growth factor 2
  • NUDT6
  • Prostatropin

Background

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. A variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration. FGF basic also modulates embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

  1. Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
  2. Chen, C.H. et al. (2004) Curr. Vasc. Pharmacol. 2:33.
  3. Mohammadi, M. et al. (2005) Curr. Opin. Struct. Biol. 15:506.
  4. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.

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Publications for FGF basic/FGF2 (233-FB)(283)

We have publications tested in 11 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Hamster, N/A, Porcine, Primate - Callitrix jacchus (Common Marmoset), Zebrafish.

We have publications tested in 8 applications: Binding Assay, Bioassay, Cell Culture Treatment, ELISA Standard, Expansion/Differentiation, In Vivo, cell culture, differentiation.


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Binding Assay
(1)
Bioassay
(265)
Cell Culture Treatment
(1)
ELISA Standard
(1)
Expansion/Differentiation
(1)
In Vivo
(8)
cell culture
(1)
differentiation
(1)
All Applications
Filter By Species
Human
(215)
Mouse
(33)
Rat
(10)
Bovine
(4)
Canine
(3)
Chicken
(6)
Hamster
(1)
N/A
(1)
Porcine
(4)
Primate - Callitrix jacchus (Common Marmoset)
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 283. Show All 283 Publications.
Publications using 233-FB Applications Species
J Ooi, SR Langley, X Xu, KH Utami, B Sim, Y Huang, NP Harmston, YL Tay, A Ziaei, R Zeng, D Low, F Aminkeng, RM Sobota, F Ginhoux, E Petretto, MA Pouladi Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects Cell Rep, 2019;26(9):2494-2508.e7. 2019 [PMID: 30811996]
N Muraoka, K Nara, F Tamura, H Kojima, H Yamakawa, T Sadahiro, K Miyamoto, M Isomi, S Haginiwa, H Tani, S Kurotsu, R Osakabe, S Torii, S Shimizu, H Okano, Y Sugimoto, K Fukuda, M Ieda Role of cyclooxygenase-2-mediated prostaglandin E2-prostaglandin E receptor 4 signaling in cardiac reprogramming Nat Commun, 2019;10(1):674. 2019 [PMID: 30787297] (Bioassay, Mouse) Bioassay Mouse
M Kunova Bos, A Nita, T Gregor, M Varecha, I Gudernova, B Fafilek, T Barta, N Basheer, SP Abraham, L Balek, M Tomanova, J Fialova Ku, J Bosak, D Potesil, J Zieba, J Song, P Konik, S Park, I Duran, Z Zdrahal, D Smajs, G Jansen, Z Fu, HW Ko, A Hampl, L Trantirek, D Krakow, P Krejci Fibroblast growth factor receptor influences primary cilium length through an interaction with intestinal cell kinase Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):. 2019 [PMID: 30782830] (Bioassay, Human) Bioassay Human
TS Winston, K Suddhapas, C Wang, R Ramos, P Soman, Z Ma Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells Stem Cells Int, 2019;2019(0):5654324. 2019 [PMID: 30766604] (Bioassay, Human) Bioassay Human
E Ito, S Miyagawa, M Takeda, A Kawamura, A Harada, H Iseoka, S Yajima, N Sougawa, N Mochizuki-, S Yasuda, Y Sato, Y Sawa Tumorigenicity assay essential for facilitating safety studies of hiPSC-derived cardiomyocytes for clinical application Sci Rep, 2019;9(1):1881. 2019 [PMID: 30760836] (Bioassay, Human) Bioassay Human
JH Wang, ST Huang, L Zhang, ZG Liu, RX Liang, SW Jiang, YN Jiang, XJ Yu, YC Jiang, XZ Li, PF Zhang, ZS Wen, M Zheng Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinoma Cancer Med, 2019;0(0):. 2019 [PMID: 30741466] (Bioassay, Human) Bioassay Human
S Bunda, P Heir, J Metcalf, ASC Li, S Agnihotri, S Pusch, M Yasin, M Li, K Burrell, S Mansouri, O Singh, M Wilson, A Alamsahebp, R Nejad, B Choi, D Kim, A von Deimli, G Zadeh, K Aldape CIC protein instability contributes to tumorigenesis in glioblastoma Nat Commun, 2019;10(1):661. 2019 [PMID: 30737375] (Bioassay, Human) Bioassay Human
S Ashraf, S Bell, C O'Leary, P Canning, I Micu, JA Fernandez, M O'Hare, P Barabas, H McCauley, DP Brazil, AW Stitt, JG McGeown, TM Curtis CAMKII as a therapeutic target for growth factor-induced retinal and choroidal neovascularisation JCI Insight, 2019;0(0):. 2019 [PMID: 30721154] (Bioassay, Human) Bioassay Human
JH Jo, SB Park, S Park, HS Lee, C Kim, DE Jung, SY Song Novel Gastric Cancer Stem Cell-Related Marker LINGO2 Is Associated with Cancer Cell Phenotype and Patient Outcome Int J Mol Sci, 2019;20(3):. 2019 [PMID: 30696080] (Bioassay, Human) Bioassay Human
M Riera, A Patel, B Corcostegu, S Chang, B Corneo, JR Sparrow, E Pomares Generation of an induced pluripotent stem cell line (FRIMOi002-A) from a retinitis pigmentosa patient carrying compound heterozygous mutations in USH2A gene Stem Cell Res, 2019;35(0):101386. 2019 [PMID: 30685615] (Bioassay, Human) Bioassay Human
Show All 283 Publications.

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FAQs for FGF basic/FGF2 (233-FB). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

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Bioinformatics

Gene Symbol FGF2
Uniprot