Recombinant Human Erythropoietin (Tissue Culture Grade)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human Erythropoietin (Tissue Culture Grade) Summary

Details of Functionality
Calibrated against the second international reference preparation of erythropoietin. Annable, L. et al. (1972) Bull. Hlth. Org. 47:99. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.015-0.075 units/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Erythropoietin protein
Ala28-Arg193
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
EPO
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
37 kDa, reducing conditions
Publications
Read Publications using
287-TC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Instructions
Reconstitute at 500 U/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Erythropoietin (Tissue Culture Grade)

  • ECYT5
  • EP
  • EPO
  • epoetin
  • Erythropoietin
  • MGC138142
  • MVCD2

Background

Erythropoietin (Epo) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of Epo is required for biological activities in vivo (4). Mature human Epo shares 75% - 84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6 - 8). Epo promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the Epo receptor (Epo R) (8, 9). Epo R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of Epo R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating Epo allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce Epo production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). Epo additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

  1. Koury, M.J. (2005) Exp. Hematol. 33:1263.
  2. Jacobs, K. et al. (1985) Nature 313:806.
  3. Wen, D. et al. (1993) Blood 82:1507.
  4. Tsuda E., et al. (1990) Eur. J. Biochem. 188:405.
  5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
  6. Eckardt, K.U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
  7. Sharples, E.J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
  8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
  9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
  10. Acs, G. et al. (2001) Cancer Res. 61:3561.
  11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
  12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
  13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.

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Publications for Erythropoietin (287-TC)(28)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: Bioassay, ELISA (Control).


Filter By Application
Bioassay
(27)
ELISA (Control)
(1)
All Applications
Filter By Species
Human
(22)
Mouse
(5)
Rat
(1)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using 287-TC Applications Species
FS Kittur, Y Lin, E Arthur, CY Hung, PA Li, DC Sane, J Xie Recombinant asialoerythropoetin protects HL-1 cardiomyocytes from injury via suppression of Mst1 activation Biochem Biophys Rep, 2019;17(0):157-168. 2019 [PMID: 30671548] (Bioassay, Mouse) Bioassay Mouse
L Cyganek, M Tiburcy, K Sekeres, K Gerstenber, H Bohnenberg, C Lenz, S Henze, M Stauske, G Salinas, WH Zimmermann, G Hasenfuss, K Guan Deep phenotyping of human induced pluripotent stem cell-derived atrial and ventricular cardiomyocytes JCI Insight, 2018;3(12):. 2018 [PMID: 29925689] (Bioassay, Human) Bioassay Human
MC Allenby, A Tahlawi, JCF Morais, K Li, N Panoskalts, A Mantalaris Ceramic Hollow Fibre Constructs for Continuous Perfusion and Cell Harvest from 3D Hematopoietic Organoids Stem Cells Int, 2018;2018(0):6230214. 2018 [PMID: 29760729] (Bioassay, Human) Bioassay Human
SY Han, EM Lee, HS Choi, BH Chun, EJ Baek The effects of plasma gelsolin on human erythroblast maturation for erythrocyte production Stem Cell Res, 2018;29(0):64-75. 2018 [PMID: 29597129] (Bioassay, Human) Bioassay Human
HJ Yoon, X Zhang, MG Kang, GJ Kim, SY Shin, SH Baek, BN Lee, SJ Hong, JT Kim, K Hong, H Bae Cytotoxicity Evaluation of Turmeric Extract Incorporated Oil-in-Water Nanoemulsion Int J Mol Sci, 2018;19(1):. 2018 [PMID: 29342111] (Bioassay, Human) Bioassay Human
S Miao, SM Wang, X Cheng, YF Li, QS Zhang, G Li, SQ He, XP Chen, P Wu Erythropoietin promoted the proliferation of hepatocellular carcinoma through hypoxia induced translocation of its specific receptor Cancer Cell Int., 2017;17(0):119. 2017 [PMID: 29238266] (Bioassay, Human) Bioassay Human
N Rahman, PM Brauer, L Ho, T Usenko, M Tewary, JC Zúñiga-Pfl, PW Zandstra Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence Nat Commun, 2017;8(0):15380. 2017 [PMID: 28541275] (Bioassay, Human) Bioassay Human
E Arthur, FS Kittur, Y Lin, CY Hung, DC Sane, J Xie Plant-Produced Asialo-Erythropoietin Restores Pancreatic Beta-Cell Function by Suppressing Mammalian Sterile-20-like Kinase (MST1) and Caspase-3 Activation Front Pharmacol, 2017;8(0):208. 2017 [PMID: 28469576] (Bioassay, Rat) Bioassay Rat
Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system Gene Ther., 2016;23(10):699-707. 2016 [PMID: 27356951] (ELISA (Control), Human) ELISA (Control) Human
Canli O, Alankus Y, Grootjans S, Vegi N, Hultner L, Hoppe P, Schroeder T, Vandenabeele P, Bornkamm G, Greten F Glutathione peroxidase 4 prevents necroptosis in mouse erythroid precursors. Blood, 2016;127(1):139-48. 2016 [PMID: 26463424] (Bioassay, Mouse) Bioassay Mouse
Show All 28 Publications.

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Bioinformatics

Gene Symbol EPO
Uniprot