Recombinant Human Erythropoietin/EPO (Ultrapure) Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Erythropoietin/EPO (Ultrapure) Protein, CF Summary

Details of Functionality
Calibrated against the second international reference preparation of erythropoietin. Annable, L. et al. (1972) Bull. Hlth. Org. 47:99. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.02-0.12 units/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Erythropoietin/EPO protein
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
EPO
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
37 kDa, reducing conditions
Publications
Read Publications using
286-EP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Erythropoietin/EPO (Ultrapure) Protein, CF

  • ECYT5
  • EP
  • EPO
  • epoetin
  • Erythropoietin
  • MGC138142
  • MVCD2

Background

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of EPO is required for biological activities in vivo (4). Mature human EPO shares 75%-84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R) (8, 9). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

  1. Koury, M. J. (2005) Exp. Hematol. 33:1263.
  2. Jacobs, K. et al. (1985) Nature 313:806.
  3. Wen, D. et al. (1993) Blood 82:1507.
  4. Tsuda E., et al. (1990) Eur. J. Biochem. 188:405.
  5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
  6. Eckardt, K. U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35: Suppl. 3:13.
  7. Sharples, E. J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
  8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
  9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
  10. Acs, G. et al. (2001) Cancer Res. 61:3561.
  11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
  12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
  13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.

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Publications for Erythropoietin/EPO (286-EP)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA (Control), In Vivo.


Filter By Application
Bioassay
(3)
Cell Culture
(1)
ELISA (Control)
(1)
In Vivo
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(3)
All Species
Showing Publications 1 - 6 of 6.
Publications using 286-EP Applications Species
T Fukushima, Y Tanaka, FK Hamey, CH Chang, T Oki, S Asada, Y Hayashi, T Fujino, T Yonezawa, R Takeda, KC Kawabata, T Fukuyama, T Umemoto, K Takubo, H Takizawa, S Goyama, Y Ishihama, H Honda, B Göttgens, T Kitamura Discrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium Cell Rep, 2019;29(12):4144-4158.e7. 2019 [PMID: 31851939] (Cell Culture, Mouse) Cell Culture Mouse
RA Sheldon, C Windsor, BS Lee, O Arteaga Ca, DM Ferriero Erythropoietin Treatment Exacerbates Moderate Injury after Hypoxia-Ischemia in Neonatal Superoxide Dismutase Transgenic Mice Dev. Neurosci., 2017;0(0):. 2017 [PMID: 28445874] (In Vivo, Mouse) In Vivo Mouse
Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system Gene Ther., 2016;23(10):699-707. 2016 [PMID: 27356951] (ELISA (Control), Human) ELISA (Control) Human
Kirouac DC, Ito C, Csaszar E, Roch A, Yu M, Sykes EA, Bader GD, Zandstra PW Dynamic interaction networks in a hierarchically organized tissue. Mol. Syst. Biol., 2010;6(0):417. 2010 [PMID: 20924352] (Bioassay, Human) Bioassay Human
Yilmaz OH, Kiel MJ, Morrison SJ SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity. Blood, 2005;107(3):924-30. 2005 [PMID: 16219798] (Bioassay, Mouse) Bioassay Mouse
Barcena A, Park SW, Banapour B, Muench MO, Mechetner E Expression of Fas/CD95 and Bcl-2 by primitive hematopoietic progenitors freshly isolated from human fetal liver. Blood, 1996;88(6):2013-25. 1996 [PMID: 8822920] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol EPO
Uniprot