Recombinant Human BAFF/BLyS/TNFSF13B Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human BAFF/BLyS/TNFSF13B Protein Summary

Additional Information
A New rh BAFF is Now Available! The new protein is competitively priced and has no tag!
Details of Functionality
Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.4-2 ng/mL in the presence of goat anti‑mouse IgM µ chain.
Source
Mouse myeloma cell line, NS0-derived human BAFF/BLyS/TNFSF13B protein
MHHHHHHHHHH GGGSGGGSGGGS IEGR Human BAFF
(Ala134-Leu285)
Accession # Q9Y275
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
TNFSF13B
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
22-29 kDa, reducing conditions
Publications
Read Publications using
2149-BF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BAFF/BLyS/TNFSF13B Protein

  • ApoL related ligand TALL-1
  • B lymphocyte stimulator
  • BAFF
  • BAFFB-cell activating factor
  • B-cell-activating factor
  • BLyS
  • BLYSB-lymphocyte stimulator
  • CD257 antigen
  • CD257
  • Dendritic cell-derived TNF-like molecule
  • DTL
  • TALL1
  • TALL-1delta BAFF
  • TALL1Delta4 BAFF
  • THANK
  • TNF- and APOL-related leukocyte expressed ligand 1
  • TNF and ApoL-related leukocyte expressed ligand 1
  • TNF homolog that activates apoptosis
  • TNFSF13B
  • TNFSF20
  • tumor necrosis factor (ligand) superfamily, member 13b
  • tumor necrosis factor (ligand) superfamily, member 20
  • tumor necrosis factor ligand superfamily member 13B
  • tumor necrosis factor superfamily, member 13b
  • tumor necrosis factor-like protein ZTNF4
  • ZTNF4

Background

B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein in the TNF ligand superfamily. It is involved in multiple aspects of immune system regulation, particularly towards B cells (1, 2). Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain (3, 4). Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF. It can be expressed as a homotrimer or as a heteromer in association with the related TNFSF member APRIL (4, 5). A 18 kDa fragment containing the TNF-like domain can be released by proteolysis between Arg133 and Ala134 (4). Soluble BAFF is stored intracellularly in neutrophils and released upon inflammatory stimulation (6). Alternative splicing generates an isoform termed deltaBAFF that lacks 19 aa between the proteolytic cleavage site and the TNF-like domain. deltaBAFF can form heteromers with BAFF and negatively regulates BAFF function (7). BAFF is produced by many hematopoietic cell types including by monocytes, macrophages, neutrophils, dendritic cells, and T cells and also by adipocytes (1, 2, 8). Both BAFF and APRIL are functional ligands for the TNF receptor superfamily members BCMA and TACI, and BAFF additionally binds and signals through BAFF R (9, 10). All three receptors are primarily expressed by B cells (10). BAFF plays a critical role in the development and survival of B lineage cells (2, 11, 12). Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity (13). Soluble BAFF is elevated in B cell malignancies, autoimmunity, and other immune disorders (1). In addition, BAFF co-stimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells (14-16). BAFF also promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages (17).
  1. Lied, G.A. and A. Berstad (2011) Scand. J. Immunol. 73:1.
  2. Mackay, F. et al. (2010) Immunol. Rev. 237:205.
  3. Moore, P.A. et al. (1999) Science 285:260.
  4. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  5. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  6. Scapini, P. et al. (2003) J. Exp. Med. 197:297.
  7. Gavin, A.L. et al. (2003) J. Biol. Chem. 278:38220.
  8. Alexaki, V.-I. et al. (2009) J. Immunol. 183:5948.
  9. Yu, G. et al. (2000) Nat. Immunol. 1:252.
  10. Thompson, J.S. et al. (2001) Science 293:2108.
  11. Schiemann, B. et al. (2001) Science 293:2111.
  12. Litinskiy, M.B. et al. (2002) Nat. Immunol. 3:822.
  13. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  14. Huard, B. et al. (2001) J. Immunol. 167:6225.
  15. Sutherland, A.P.R. et al. (2005) J. Immunol. 174:5537.
  16. Walters, S. et al. (2009) J. Immunol. 182:793.
  17. Chang, S.K. et al. (2006) Blood 108:2687.

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2149-BF
Species: Hu
Applications: Bioactivity

Publications for BAFF/BLyS/TNFSF13B (2149-BF)(18)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Bioassay, Flow Cytometry, Surface Plasmon Resonance (SPR.


Filter By Application
Bioassay
(16)
Flow Cytometry
(1)
Surface Plasmon Resonance (SPR
(1)
All Applications
Filter By Species
Human
(18)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using 2149-BF Applications Species
M Mulazzani, M Huber, S Borchard, S Langer, B Angele, E Schuh, E Meinl, M Dreyling, T Birnbaum, A Straube, U Koedel, L von Baumga APRIL and BAFF: novel biomarkers for central nervous system lymphoma J Hematol Oncol, 2019;12(1):102. 2019 [PMID: 31615554] (Bioassay, Human) Bioassay Human
R Wilson, M Makuch, AK Kienzler, J Varley, J Taylor, M Woodhall, J Palace, MI Leite, P Waters, SR Irani Condition-dependent generation of aquaporin-4 antibodies from circulating B cells in neuromyelitis optica Brain, 2018;141(4):1063-1074. 2018 [PMID: 29447335] (Bioassay, Human) Bioassay Human
AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 2018;0(0):1-43. 2018 [PMID: 29436901] (Surface Plasmon Resonance (SPR, Human) Surface Plasmon Resonance (SPR Human
J Heremans, JE Garcia-Per, E Turro, SM Schlenner, I Casteels, R Collin, F de Zegher, D Greene, S Humblet-Ba, S Lesage, P Matthys, CJ Penkett, K Put, K Stirrups, C Thys, C Van Geet, E Van Nieuwe, C Wouters, I Meyts, K Freson, A Liston, Abnormal differentiation of B cells and megakaryocytes in Roifman syndrome J. Allergy Clin. Immunol., 2018;0(0):. 2018 [PMID: 29391254] (Flow Cytometry, Human) Flow Cytometry Human
HD Brightbill, E Suto, N Blaquiere, N Ramamoorth, S Sujatha-Bh, EB Gogol, GM Castanedo, BT Jackson, YC Kwon, S Haller, J Lesch, K Bents, C Everett, PB Kohli, S Linge, L Christian, K Barrett, A Jaochico, LM Berezhkovs, PW Fan, Z Modrusan, K Veliz, MJ Townsend, J DeVoss, AR Johnson, R Godemann, WP Lee, CD Austin, BS McKenzie, JA Hackney, JJ Crawford, ST Staben, MH Alaoui Ism, LC Wu, N Ghilardi NF-?B inducing kinase is a therapeutic target for systemic lupus erythematosus Nat Commun, 2018;9(1):179. 2018 [PMID: 29330524] (Bioassay, Human) Bioassay Human
D Brach, D Johnston-B, A Drew, T Lingaraj, V Motwani, NM Warholic, I Feldman, C Plescia, JJ Smith, RA Copeland, H Keilhack, E Chan-Peneb, SK Knutson, SA Ribich, A Raimondi, MJ Thomenius EZH2 inhibition by tazemetostat results in altered dependency on B-cell activation signaling�in DLBCL Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28835384] (Bioassay, Human) Bioassay Human
VJ Sindhava, MA Oropallo, K Moody, M Naradikian, LE Higdon, L Zhou, A Myles, N Green, K Nündel, W Stohl, AM Schmidt, W Cao, S Dorta-Estr, T Kambayashi, A Marshak-Ro, MP Cancro A TLR9-dependent checkpoint governs B cell responses to DNA-containing antigens J. Clin. Invest, 2017;0(0):. 2017 [PMID: 28346226] (Bioassay, Human) Bioassay Human
Mamara, Antigoni, Germenis, Anastasi, Kompoti, Maria, Palassopoulou, Maria, Mandala, Eudokia, Banti, Anastasi, Giannakoulas, Nikolaos, Speletas, Matthaio TACI expression and signaling in chronic lymphocytic leukemia. J Immunol Res, 2015;2015(0):478753. 2015 [PMID: 25950010] (Bioassay, Human) Bioassay Human
Chinen Y, Kuroda J, Shimura Y, Nagoshi H, Kiyota M, Yamamoto-Sugitani M, Mizutani S, Sakamoto N, Ri M, Kawata E, Kobayashi T, Matsumoto Y, Horiike S, Iida S, Taniwaki M Phosphoinositide protein kinase PDPK1 is a crucial cell signaling mediator in multiple myeloma. Cancer Res, 2014;74(24):7418-29. 2014 [PMID: 25269480] (Bioassay, Human) Bioassay Human
Caro-Maldonado, Alfredo, Wang, Ruoning, Nichols, Amanda G, Kuraoka, Masayuki, Milasta, Sandra, Sun, Lillian, Gavin, Amanda L, Abel, E Dale, Kelsoe, Garnett, Green, Douglas, Rathmell, Jeffrey Metabolic reprogramming is required for antibody production that is suppressed in anergic but exaggerated in chronically BAFF-exposed B cells. J Immunol, 2014;192(8):3626-36. 2014 [PMID: 24616478] (Bioassay, Human) Bioassay Human
Show All 18 Publications.

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Bioinformatics

Gene Symbol TNFSF13B
Uniprot