Recombinant Human AMSH/STAMBP (Sf 21-expressed) Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human AMSH/STAMBP (Sf 21-expressed) Protein, CF Summary

Details of Functionality
Recombinant Human AMSH is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human AMSH concentration of 100-500 nM.  AMSH activity is partially or fully inhibited by DTT at concentrations above 1 mM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human AMSH/STAMBP protein
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
STAMBP
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
E-548B in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl,10% Glycerol (v/v), 1 mM TCEP

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human AMSH/STAMBP (Sf 21-expressed) Protein, CF

  • AMSH
  • AMSHAssociated molecule with the SH3 domain of STAM
  • EC 3.1.2.15
  • EC 3.4.19.-
  • Endosome-associated ubiquitin isopeptidase
  • MGC126516
  • MGC126518
  • STAM binding protein
  • STAM-binding protein
  • STAMBP

Background

AMSH (STAM binding protein) is a zinc metalloprotease belonging to the JAMM (JAB1/MPN/Mov34) family of deubiquitinating enzymes (DUBs). This enzyme functions at the endosome, where it is involved in the sorting of various cell-surface receptors to lysozomes. AMSH cleaves K63-linked but not K48-linked polyubiquitin chains, and the in vitro activity of AMSH is greatly increased in the presence of STAM protein. This recombinant protein is full-length.
  1. Agromayor, M. and Martin-Serrano, J. (2006) J. Biol. Chem 281:23083
  2. Kim, M.S., et al. (2006) Biochem. Biophys. Res Comm. 351:612
  3. McCullough, J., et al. (2006) Current Biology 16:160
  4. Sato, Y., et al. (2008) Nature. 455:358

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Publications for AMSH/STAMBP (E-548B)(4)

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Bioinformatics

Gene Symbol STAMBP
Uniprot