Recombinant Human Activin B Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Activin B Protein, CF Summary

Details of Functionality
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.3‑1.5 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Activin B protein
Gly293-Ala407
Accession #
N-terminal Sequence
Gly293
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
INHBB
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12.4 kDa, reducing conditions
Publications
Read Publications using
659-AB/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute 5 µg vials at 50 µg/mL in sterile PBS. Reconstitute 25 µg or larger vials at 100 µg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Activin B Protein, CF

  • Activin B
  • INHBB
  • Inhibin, Beta B
  • Inhibin, Beta-2

Background

Activins and inhibins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins that were originally purified from gonadal fluids as proteins that stimulated or inhibited, respectively, pituitary follicle stimulating hormone (FSH) release. These proteins have since been shown to have a wide range of biological activities including: mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and reproductive physiology. Activins/inhibins are produced as precursor proteins with an amino-terminal propeptide that is cleaved to release the carboxy-terminal bioactive ligands. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha  subunit and one of the various beta  subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and  Xenopus beta D) have been cloned. The activin/inhibin nomenclature reflects the subunit composition of the proteins: Activin A ( beta A - beta A), activin B ( beta - beta B), Activin AB ( beta A - beta B), inhibin A ( alpha - beta A) and inhibin B ( alpha - beta B). Little is known about the contribution of the other beta  subunits to activin or inhibin formation and biology. At the amino acid sequence level, the mature human beta B subunit is greater than 98% identical to mouse beta B, while the human and mouse alpha  subunits share approximately 80% identity. Similar to other TGF-beta  family members, activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated as type I and type II. Two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B) have been identified. Activin binds directly to Act RII, the complex then associates with Act RI and initiates signaling. Besides activins, Act RII has been shown to bind certain other TGF-beta superfamily members. Inhibin A has been shown to bind with low-affinity to Act RII. The existence of a distinct inhibin-specific receptor and/or signal transduction pathway has been hypothesized.

  1. Woodruff, T.K. (1998) Biochemical Pharmacology 55:953.
  2. Ying, S.Y. et al. (1997) Proc. Soc. Exp. Biol. Med. 214:114.

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Publications for Activin B/Inhibin beta B (659-AB/CF)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 3 applications: Bioassay, In Vivo, Surface Plasmon Resonance.


Filter By Application
Bioassay
(12)
In Vivo
(1)
Surface Plasmon Resonance
(2)
All Applications
Filter By Species
Human
(11)
Mouse
(2)
Rat
(1)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using 659-AB/CF Applications Species
L Tengroth, J Arebro, O Larsson, C Bachert, SK Georén, LO Cardell Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps Sci Rep, 2018;8(1):1561. 2018 [PMID: 29367682] (Bioassay, Human) Bioassay Human
A Kita, A Kasamatsu, D Nakashima, Y Endo-Sakam, S Ishida, T Shimizu, Y Kimura, I Miyamoto, S Yoshimura, M Shiiba, H Tanzawa, K Uzawa Activin B Regulates Adhesion, Invasiveness, and Migratory Activities in Oral Cancer: a Potential Biomarker for Metastasis J Cancer, 2017;8(11):2033-2041. 2017 [PMID: 28819404] (Bioassay, Human) Bioassay Human
E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada Activin A more prominently regulates muscle mass in primates than does GDF8 Nat Commun, 2017;8(0):15153. 2017 [PMID: 28452368] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Y Kanamori, M Murakami, M Sugiyama, O Hashimoto, T Matsui, M Funaba IL-1? transcriptionally activates hepcidin by inducing C/EBP? expression in hepatocytes J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28438835] (Bioassay, Human) Bioassay Human
CS Stephens, PA Johnson Occludin expression and regulation in small follicles of the layer and broiler breeder hen Gen. Comp. Endocrinol, 2017;0(0):. 2017 [PMID: 28238709] (Bioassay, Chicken) Bioassay Chicken
Regulation of hepcidin expression by inflammation-induced activin B Sci Rep, 2016;6(0):38702. 2016 [PMID: 27922109] (Bioassay, Human) Bioassay Human
Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El-Sayed K, Ehnert S, Nussler A, Fandrich F, Gieseler F Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta. PLoS ONE, 2015;10(2):e0118097. 2015 [PMID: 25707005] (Bioassay, Human) Bioassay Human
Tamminen J, Yin M, Ronty M, Sutinen E, Pasternack A, Ritvos O, Myllarniemi M, Koli K Overexpression of activin-A and -B in malignant mesothelioma - attenuated Smad3 signaling responses and ERK activation promote cell migration and invasive growth. Exp Cell Res, 2015;332(1):102-15. 2015 [PMID: 25557874] (Bioassay, Human) Bioassay Human
Carrancio S, Markovics J, Wong P, Leisten J, Castiglioni P, Groza M, Raymon H, Heise C, Daniel T, Chopra R, Sung V An activin receptor IIA ligand trap promotes erythropoiesis resulting in a rapid induction of red blood cells and haemoglobin. Br J Haematol, 2014;165(6):870-82. 2014 [PMID: 24635723] (Bioassay, Human) Bioassay Human
Kadaja, Meelis, Keyes, Brice E, Lin, Mingyan, Pasolli, H Amalia, Genander, Maria, Polak, Lisa, Stokes, Nicole, Zheng, Deyou, Fuchs, Elaine SOX9: a stem cell transcriptional regulator of secreted niche signaling factors. Genes Dev, 2014;28(4):328-41. 2014 [PMID: 24532713] (In Vivo, Mouse) In Vivo Mouse
Show All 15 Publications.

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Bioinformatics

Gene Symbol INHBB
Entrez
Uniprot