RC74 Antibody


Western Blot: RC74 Antibody [NB100-589] - Nuclear extract (25 mcg for WB in A; 5 mg for IP in B) from HeLa cells. Affinity purified rabbit anti-RC74 antibody used at 0.05 mcg/ml for WB in A and at 4 mcg/5 mg extract ...read more
Immunohistochemistry: RC74 Antibody [NB100-589] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RC74 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB

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RC74 Antibody Summary

A synthetic peptide that maps to a region between residues 150 and 200 of human Related to CPSF subunits 74 kDa using the numbering given in TrEMBL entry Q8TB16 (GeneID 55756).
Immunogen affinity purified
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  • Western Blot 1:10000-1:25000
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:2500
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 1-4 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000-1:25,000 Immunoprecipitation: 1-4 ug/mg lysate Histo/cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Positive Control
HeLa Lysate (NB800-PC9)
RC74 Lysate (NBL1-12012)

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for RC74 Antibody

  • CPSF2L
  • FLJ10871
  • INT9
  • integrator complex subunit 9
  • Protein related to CPSF subunits of 74 kDa
  • RC74
  • RC-74


Formation of the mature 3' ends of the vast majority of cellular mRNAs occurs through cleavage and polyadenylation and requires a cleavage and polyadenylation specificity factor (CPSF) containing, among other proteins, CPSF-73 and CPSF-100. RC-74 is an exclusively nuclear protein found in HeLa cells that has similar characteristics to CPSF-100. Like the complex that forms between CPSF-100 and CPSF-73, studies show that RC-74 may form a similar complex with RC-68 that is independent from the CPSF complex. This RC-68 / RC-74 complex may be involved in a pre-mRNA processing event other than cleavage and polyadenylation, such as the 3' end processing of a distinct subset of cellular pre-mRNAs encoding proteins required for G(1) progression and entry into S phase.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies


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Gene Symbol INTS9