RC74 Antibody

Images

 
Western Blot: RC74 Antibody [NB100-589] - Nuclear extract (25 mcg for WB in A; 5 mg for IP in B) from HeLa cells. Affinity purified rabbit anti-RC74 antibody used at 0.05 mcg/ml for WB in A and at 4 mcg/5 mg extract ...read more
Immunohistochemistry: RC74 Antibody [NB100-589] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RC74 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB

Product Details

Summary
Product Discontinued
View other related RC74 Primary Antibodies

Order Details


    • Catalog Number
      NB100-589
    • Availability
      Product Discontinued

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RC74 Antibody Summary

Immunogen
A synthetic peptide that maps to a region between residues 150 and 200 of human Related to CPSF subunits 74 kDa using the numbering given in TrEMBL entry Q8TB16 (GeneID 55756).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
INTS9
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:25000
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:2500
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 1-4 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000-1:25,000 Immunoprecipitation: 1-4 ug/mg lysate Histo/cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Positive Control
HeLa Lysate (NB800-PC9)
RC74 Lysate (NBL1-12012)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RC74 Antibody

  • CPSF2L
  • FLJ10871
  • INT9
  • integrator complex subunit 9
  • Protein related to CPSF subunits of 74 kDa
  • RC74
  • RC-74

Background

Formation of the mature 3' ends of the vast majority of cellular mRNAs occurs through cleavage and polyadenylation and requires a cleavage and polyadenylation specificity factor (CPSF) containing, among other proteins, CPSF-73 and CPSF-100. RC-74 is an exclusively nuclear protein found in HeLa cells that has similar characteristics to CPSF-100. Like the complex that forms between CPSF-100 and CPSF-73, studies show that RC-74 may form a similar complex with RC-68 that is independent from the CPSF complex. This RC-68 / RC-74 complex may be involved in a pre-mRNA processing event other than cleavage and polyadenylation, such as the 3' end processing of a distinct subset of cellular pre-mRNAs encoding proteins required for G(1) progression and entry into S phase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional RC74 Products

Bioinformatics Tool for RC74 Antibody (NB100-589)

Discover related pathways, diseases and genes to RC74 Antibody (NB100-589). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol INTS9
Entrez
Uniprot