RBFOX3/NeuN Antibody (1B7)

Images

 
Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - SHSY5Y cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The ...read more
Immunohistochemistry-Frozen: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - IHC-Fr analysis of a 4% PFA fixed mouse spinal cord tissue section using RBFOX3/NeuN antibody (clone 1B7) at 1:500 dilution with overnight 4C ...read more
Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - An intracellular stain was performed on U87-MG cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693AF700 (blue) and a matched isotype control (orange). Cells were ...read more
Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - Rat brain neural cultures stained with NBP1-92693 (red), a chicken polyclonal antibody to GFAP (NBP1-05198, green) and DAPI (blue). ...read more
Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - Dissociated spinal cord nuclei immunostained with anti-NeuN antibody and Alexa 488 secondary. DAPI (Blue) to stain nuclei. This image ...read more
Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - Formalin fixed rat brain stem neurons stained with to NeuN in green and counter stained with, our chicken polyclonal antibody to microtubule ...read more
Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP1-92693. Staining of mouse brain cells - Trypsin-EDTA digestion, 2% PFA fixation, primary used at 1:10 ...read more
Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - Analysis of PE conjugate of NBP1-92693. An intracellular stain was performed on SH-SY5Y cells with RBFOX3 (1B7) antibody NBP1-92693PE (blue) and a matched ...read more
Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - Using the Allophycocyanin direct conjugate An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693APC (blue) and a ...read more
Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - An intracellular stain was performed on A549 cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693AF647 (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - Using the PerCP direct conjugate An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693PCP (blue) and a matched ...read more
Flow (Intracellular): RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693AF700 (blue) and a matched isotype control (orange). Cells ...read more
Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - An intracellular stain was performed on A549 cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693APC (blue) and a matched isotype control (orange). Cells were fixed ...read more
Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - An intracellular stain was performed on A549 cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693F (blue) and a matched isotype control (orange). Cells were fixed ...read more
Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693AF647 (blue) and a matched isotype control (orange). Cells were ...read more
RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - Analysis of whole brain tissue lysates using mouse mAb to FOX3/NeuN NBP1-92693, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
1B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

RBFOX3/NeuN Antibody (1B7) Summary

Immunogen
N-terminal 100 amino acids of human FOX3 as expressed in and purified from E. coli
Localization
Nucleus. Cytoplasm.
Marker
Neuronal Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RBFOX3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Frozen 1:400
  • Immunohistochemistry-Paraffin
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
This RBFOX3/Neun (1B7) antibody is useful for ICC and Western blot, where bands can be seen at 46 and 48 kDa. Use in IHC-frozen sections was reported in the scientific literature (PMID: 23776455). FLOW application was reported by Dr Xingrao Ke from Dept. of Pediatrics at Medical College of Wisconsin - see the product review at the datasheet of NBP1-92693AF700 for methodology details. This antibody is CyTOF ready.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-92693 in the following applications:

Publications
Read Publications using
NBP1-92693 in the following applications:

Reactivity Notes

Human, bovine, porcine, mouse, rat and other mammals.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% Glycerol
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RBFOX3/NeuN Antibody (1B7)

  • FLJ56884
  • FLJ58356
  • Fox-1 homolog C
  • FOX3
  • FOX-3
  • hexaribonucleotide binding protein 3
  • HRNBP3
  • NeuN flow cytometry
  • NeuN
  • neuronal nuclei
  • RBFOX3
  • RNA binding protein fox-1 homolog 3
  • RNA binding protein, fox-1 homolog (C. elegans) 3

Background

RBFOX3/NeuN (RNA binding protein FOX-1 homolog 3/ Neuronal nuclei) is a RNA-binding protein localized in the nucleus as well as cytoplasm that regulates alternative splicing events. It is a member of RNA-binding FOX (RBFOX) family of splicing regulators which comprises RBFOX1 (Fox-1 or A2BP1), RBFOX2 (Fox-2 or RBM9), and RBFOX3 (Fox-3, HRNBP3, or NeuN). All of these Fox protein have single central RNA recognition motif RNA-binding domain which detects sequence (U)GCAUG observed within introns flanking alternative exons. (U)GCAUG motif position corresponding to the alternative exon dictates RBFOX's effects on splicing process. A motif localized upstream from alternative exon generally repress RBFOX-dependent exon inclusion, whereas, a downstream stream motif will usually promote inclusion. Neurons, heart, and muscle express RBFOX 1, whereas, RBFOX3 is limited to neurons only, and RBFOX2 is expressed in these tissues alongwith expression in embryos, hematopoietic cells, and embryonic stem cells. Defects in alternative splicing are known to result in neurological and neuromuscular diseases such as frontotemporal dementia and myotonic dystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RBFOX3/NeuN Antibody (NBP1-92693)(5)

Reviews for RBFOX3/NeuN Antibody (NBP1-92693) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NBP1-92693:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
 RBFOX3/NeuN NBP1-92693
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reviewed by:
Anonymous
Mouse 01/26/2018
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Summary

Sample TestedDissociated spinal cord nuclei
SpeciesMouse
Lot082317
 
reviewed by:
Anonymous
IHC-Fr Mouse 05/11/2016
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse spinal cord
SpeciesMouse
Lot42514

Blocking

Blocking DetailsPBS BSA 2% Triton 0.4%, 1h, RT

Primary Anitbody

Dilution Ratio1/500, overnight, 4C, PBS BSA 1% Triton 0.2%

Secondary Antibody

Secondary DescriptionGoat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 594
Secondary Manufacturer Cat#A11032
Secondary Concentration1/500, RT, 1h, PBS BSA 1% Triton 0.2%

Details

Detection NotesOlympus BX63
Fixation DetailsPFA 4%
Wash DescriptionPBS

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RBFOX3/NeuN Antibody (NBP1-92693) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-92693AF405
Alexa Fluor 488 Labeled NBP1-92693AF488
Alexa Fluor 647 Labeled NBP1-92693AF647
Alexa Fluor 700 Labeled NBP1-92693AF700
Allophycocyanin Labeled NBP1-92693APC
DyLight 350 Labeled NBP1-92693UV
DyLight 405 Labeled NBP1-92693V
DyLight 488 Labeled NBP1-92693G
DyLight 680 Labeled NBP1-92693FR
DyLight 755 Labeled NBP1-92693IR
FITC Labeled NBP1-92693F
PE Labeled NBP1-92693PE

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Bioinformatics Tool for RBFOX3/NeuN Antibody (NBP1-92693)

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Diseases for RBFOX3/NeuN Antibody (NBP1-92693)

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Blogs on RBFOX3/NeuN.

RBFOX3: Binding RNA, like a FOX
NeuN is a RNA-binding protein that modulates alternative splicing and is localized both to the nucleus and cytoplasm. It is a member of the RNA-binding FOX (RBFOX) family of splicing regulators which includes RBFOX1 (Fox-1/A2BP1) and RBFOX2 (Fox-2/RBM...  Read full blog post.

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Recent Reviews

4.5
5
1
4
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0
2
0
1
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Anonymous
01/26/2018
Application:
Species: Mouse

 
Anonymous
05/11/2016
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol RBFOX3
Entrez
Uniprot