RBBP4/RbAp48 Antibody

Images

 
Western Blot: RbAp48 Antibody [NB120-3536] - Figure 1 shows immunolocalization of RbAp48 in HeLa cells using NB120-3536. Figure 2 shows a Western blot of RpAp48 on 1) Du-145 2) LNCaP and 3) Hep-G2 cell extracts using ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB120-3536
    • Availability
      Product Discontinued

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RBBP4/RbAp48 Antibody Summary

Immunogen
Synthetic peptide: MADKFAAFDDAVEEC, corresponding to amino acids 1-14 of Human RbAp 48.
Localization
Nuclear.
Specificity
NB120-3536 detects retinoblastoma protein (Rb) associated protein 48 (RbAp48) from human cells.
Clonality
Polyclonal
Host
Rabbit
Gene
RBBP4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
WB: Use at a concentration of 0.5 ug/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47.5 kDa). A larger ~60 kDa unknown protein from HeLa cell lysate is also detected. Can be blocked with <ab5014>RbAp 48 peptide</ab>Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human. Expected to cross-react with Chicken (92% identity with immunogen), Mouse (100% identity with immunogen), Xenopus laevis (92% identity with immunogen), Zebrafish (92% identity with immunogen) due to sequence homology. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RBBP4/RbAp48 Antibody

  • CAF-1 p48
  • CAF-1 subunit C
  • CAF-1C
  • CAF-I p48
  • Chromatin assembly factor 1 subunit C
  • Chromatin assembly factor I p48 subunit
  • chromatin assembly factor/CAF-1 p48 subunit
  • histone-binding protein RBBP4
  • MSI1 protein homolog
  • Nucleosome-remodeling factor subunit RBAP48
  • NURF55
  • RBAP48
  • RBBP4
  • RBBP-4
  • retinoblastoma binding protein 4
  • Retinoblastoma-binding protein 4CAF-I 48 kDa subunit
  • Retinoblastoma-binding protein p48

Background

Retinoblastoma protein (Rb) associated protein 48 (RbAp 48) is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex as are the histone deacetylase proteins (HDACs). RbAp 48 shares about 75% sequence identity with the closely related RbAp 46, and appears to be functionally related to the yeast MSI 1 protein in that overexpression of RbAp 48 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp48 has also been demonstrated to be up-regulated by the Wilms' tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp 48 have shown strong inhibition of growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RBBP4
Entrez