RB1 Antibody (7E4B8)

Images

 
Immunohistochemistry-Paraffin: RB1 Antibody (7E4B8) [NBP1-51527] - Immunohistochemical staining of paraffin-embedded human normal esophagus (A) and stomach (B) tissue, showing nucleus localization using Rb mouse mAb ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC, IHC-P
Clone
7E4B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RB1 Antibody (7E4B8) Summary

Immunogen
Purified recombinant fragment of human RB expressed in E. Coli.
Marker
Nuclear Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RB1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for RB1 Antibody (7E4B8)

  • OSRC
  • osteosarcoma
  • p105-Rb
  • pp110
  • pRb
  • RB
  • RB1
  • retinoblastoma 1
  • retinoblastoma suspectibility protein
  • retinoblastoma-associated protein

Background

Rb (retinoblastoma 1) is a 105 kD nuclear protein containing RB-associated protein A and B domains. Rb acts as a transcriptional negative regulator of G1-phase cell cycle progression, and sequesters E2F and other transcription factors. Rb is unphosphorylated and inactive in G0, and becomes active upon hypophosphorylation by cyclinD-cdk4/6 in early G1. Rb is inactive with hyperphosphorylated by cyclinE-cdk2 late G1. Hypophosphorylated Rb form complexes with E2F, HDAC, cyclinD-cdk4/6, cyclinE-cdk2, PU 1, ATF 2, UBF, Elf 1, and c-Abl. The Poly6146 antibody recognizes the N-terminal region of human and mouse Rb and has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence staining. Only active hypophosphorylated form complexes with E2F, HDAC, cyclinD-cdk4/6, cyclinE-cdk2, PU 1, ATF 2, UBF, Elf 1, c-Abl

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for RB1 Antibody (NBP1-51527). (Showing 1 - 1 of 1 FAQ).

  1. My research group wants to order a Retinoblastoma 1 Antibody for IHC-Paraffin. We already bought 2 antibodies from other companies and they didn't work properly. There was always a cytoplasmatic staining and no nuclear staining. I found two antibodies on your website: Retinoblastoma 1 Antibody (7E4B8) (NBP1-51527) (0.1 ml) and Retinoblastoma 1 Antibody (IF8) (NB100-65771) (0.1 ml). Can you suggest which antibody we should buy? We are using a VENTANA BenchMark ULTRA stainer, FYI. We stain human material.
    • Retinoblastoma 1 Antibody (1F8-Rb1) (NB120-3077) (0.5 ml) is one of our very high quality product that has been validated for several applications in human samples. Alternatively you can choose Retinoblastoma 1 Antibody (NB100-91947) (0.1 mg) or Retinoblastoma 1 Antibody (7E4B8) (NBP1-51527) (0.1 ml) as they both also have nice validation images for the IHC-P applications.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RB1
Entrez