Rat RAT IgG2c Kappa Isotype Control (RG2cK)


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Product Details

Reactivity RtSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF

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Rat RAT IgG2c Kappa Isotype Control (RG2cK) Summary

This Rat IgG2c immunoglobulin is useful as an isotype control for Rat IgG2c antibody.
In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1a, IgG1b, IgG3, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
Protein G purified


  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
Application Notes
Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20 degrees C or below. RAT IgG2c Kappa (?) isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: None.
0.01% Sodium Azide
Protein G purified


RAT IgG2c Kappa (?) isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain a - IgA, ? - IgG 1, 2a, 2b, 2c and u - IgM, light chain ? and ?.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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