Rat IgG, Fab2 Native Protein

Images

 
SDS-Page: Rat IgG, Fab2 Native Protein [NBP1-97015] - SDS-PAGE of Rat IgG F(ab')2 Fragment (0002). Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA, IHC, IP, PAGE
Concentration
2.0 mg/ml

Order Details

Rat IgG, Fab2 Native Protein Summary

Description
Native Rat IgG, Fab2 protein.

Source: Rat Serum

Preparation
Method
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG, anti-Rat IgG F(ab')2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.
Protein/Peptide Type
Native Protein
Purity
Immunoelectrophoresis (IEP)

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • SDS-Page 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
2.0 mg/ml
Purity
Immunoelectrophoresis (IEP)

Background

Rat IgG F(ab')2 Fragment is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. Rat IgG F(ab')2 molecules lack the Fc portion of Rat IgG and therefore receptors that bind Rat IgG Fc will not bind Rat IgG F(ab')2 molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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