RASSF1 Antibody (8E4)

Images

 
Western Blot: RASSF1 Antibody (8E4) [NBP2-03673] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: RASSF1 Antibody (8E4) [NBP2-03673] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1.
Immunohistochemistry-Paraffin: RASSF1 Antibody (8E4) [NBP2-03673] - Staining of paraffin-embedded Human prostate tissue using anti-RASSF1 mouse monoclonal antibody.
Flow Cytometry: RASSF1 Antibody (8E4) [NBP2-03673] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RASSF1 antibody, and then ...read more
Immunohistochemistry-Paraffin: RASSF1 Antibody (8E4) [NBP2-03673] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RASSF1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RASSF1 Antibody (8E4) [NBP2-03673] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RASSF1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RASSF1 Antibody (8E4) [NBP2-03673] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RASSF1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RASSF1 Antibody (8E4) [NBP2-03673] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RASSF1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RASSF1 Antibody (8E4) [NBP2-03673] - Staining of paraffin-embedded Human breast tissue using anti-RASSF1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RASSF1 Antibody (8E4) [NBP2-03673] - Staining of paraffin-embedded Human lymphoma tissue using anti-RASSF1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RASSF1 Antibody (8E4) [NBP2-03673] - Staining of paraffin-embedded Human pancreas tissue using anti-RASSF1 mouse monoclonal antibody.
Flow Cytometry: RASSF1 Antibody (8E4) [NBP2-03673] - Analysis of Jurkat cells, using anti-RASSF1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-03673
    • Availability
      Product Discontinued

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RASSF1 Antibody (8E4) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-341 of human RASSF1(NP_009113) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RASSF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RASSF1 Antibody (8E4)

  • 123F2
  • NORE2A
  • pancreas-specific ras association domain family 1 protein
  • Ras association (RalGDS/AF-6) domain family member 1
  • ras association domain-containing protein 1
  • RASSF1A
  • RDA32cardiac-specific ras association domain family 1 protein
  • REH3P21
  • tumor suppressor protein RDA32
  • WUGSC:H_LUCA12.5

Background

This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Discover related pathways, diseases and genes to RASSF1 Antibody (NBP2-03673). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for RASSF1 Antibody (NBP2-03673)

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Research Areas for RASSF1 Antibody (NBP2-03673)

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Blogs on RASSF1.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

Role of RASSF1A in Death Receptor-Dependent Apoptosis
Death-receptor apoptosis, or cell death, is essential for cellular growth regulation; its disruption is expressed in a variety of cancers. We at Novus Biologicals are one of the leading antibody suppliersfor apoptosis and cancer research groups, and t...  Read full blog post.

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Bioinformatics

Gene Symbol RASSF1
Entrez