RAS p21 Antibody (3D4)

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Immunocytochemistry/ Immunofluorescence: RAS p21 Antibody (3D4) [H00005921-M04] - Analysis of monoclonal antibody to RASA1 on HeLa cell. Antibody concentration 10 ug/ml

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
3D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RAS p21 Antibody (3D4) Summary

Immunogen
RASA1 (NP_002881 948 a.a. - 1047 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLSRDLAALHEICVAHSDELRTLSNERGAQQHVLKKLLAITELLQQKQNQYTKTNDVR
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RASA1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
It has been used for ELISA and WB.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RAS p21 Antibody (3D4)

  • CMAVM
  • DKFZp434N071
  • GAPp120GAPCM-AVM
  • GTPase-activating protein
  • p120RASGAP
  • PKWS
  • ras GTPase-activating protein 1
  • RAS p21 protein activator (GTPase activating protein) 1
  • Ras p21 protein activator
  • RASA
  • RASGAP
  • triphosphatase-activating protein

Background

RasGAP, a regulator of Ras and Rho, stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. RasGAP is a Caspase 3 substrate (1) and acts as both pro- and anti-apoptotic protein depending on the extent of its cleavage by caspases (2). At low levels of caspase activity, RasGAP is cleaved at position 455, generating an N-terminal fragment (fragment N) and a C-terminal fragment (fragment C). Fragment C alone can induce apoptosis, but this response is completely inhibited by fragment N (3). Fragment N appears to be a general blocker of apoptosis downstream of caspase activation because it inhibits caspase 9-induced cell death. At higher caspase activity, fragment N is further processed and Cleavage of fragment N abrogates its protective functions, and hence the second cleavage of RasGAP promotes apoptosis (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RASA1
Uniprot